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논문 기본 정보

자료유형
학술저널
저자정보
Min Son (Keimyung University) Oh-Sik Kwon (Keimyung University)
저널정보
계명대학교 자연과학연구소 Quantitative Bio-Science Quantitative Bio-Science Vol.35 No.2
발행연도
2016.11
수록면
53 - 60 (8page)

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이 논문의 연구 히스토리 (3)

2016

Optimization of Dextran Production from Leuconostoc mesenteroides CS-5 Isolated from Chonggak Kimchi
Min Son ,  Oh-Sik Kwon Quantitative Bio-Science 2016.11 학술저널

2014

Preliminary Study on the Dextran Production by Psychrotrophic Leuconostoc mesenteroides CS-5 Growing at a Low Temperature
Min Son ,  Oh-Sik Kwon 한국미생물학회 학술대회논문집 2014.04 학술대회자료

2013

Biochemical Characteristics of Phychrophilic Leuc. mesenteroides Strain Growing at a Low Temperature
Min Son ,  Seung-A Hwang bo ,  Oh-Sik Kwon 한국미생물학회 학술대회논문집 2013.05 학술대회자료

초록· 키워드

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The purpose of our study was to optimize the dextran production in Leuconostoc mesenteroides CS-5, which was isolated from Chonggak kimchi. To measure dextran productivity, rheological analysis and the dinitrosalicylic acid method were applied. For optimization of dextran production, 10-30% sucrose (w/v) was added to MRS medium; the highest viscosity value was found after the addition of 30% sucrose (0.153 Pa·sn), which was much higher than that found in the control (0.01 Pa·sn). However, the production of dextran by L. mesenteroides CS-5 was entirely inhibited by the addition of 17% maltose (w/v) to medium containing 30% sucrose. A subsequent test with skim milk revealed that most vigorous dextran production occurred during cultivation at 22°C with 1.5% skim milk - 0.30 Pa · s<SUP>n</SUP> (compared to 0.22 Pa · s<SUP>n</SUP> at 25°C). L. mesenteroides CS-5 is regarded as a facultative psychrophile (cold-loving bacteria). The addition of CaCl₂ increased the viscosity of the medium, owing to the production of dextran, by more than two-fold. The optimum concentration of CaCl₂ was determined to be 0.01% (w/v) which resulted in a viscosity value of 1.35 Pa · s<SUP>n</SUP>. Comparative analysis was performed using dinitrosalicylic acid tests to measure amounts of dextran according to absorbance (O.D.) at 550 nm. The highest dextran production occurred within 48 hr of incubation when using additions of 1.5% skim milk and 0.01% CaCl₂ (113.24±0.80 and 112.13±0.2, respectively). In an attempt to enhance dextran production further, the number of cells inoculated into the optimized medium was increased from 109 to 1010 and 1011 cells per 10 ml. As expected, the concentration of dextran was significantly increased by the inoculation of more cells. For 36-hr incubation, values of O. D. produced by inoculation of 1.0×1011 cells reached 123.75±0.25 (30% sucrose), 121.65±0.38 (1.5% skim milk), and 119.80±0.28 (0.01% CaCl₂); compared to 55.40±0.44 (30% sucrose), 76.83±0.06 (1.5% skim milk), and 119.80±0.28 (0.01% CaCl₂) when 1010 cells were used. During 48-hr incubation, the highest dextran production was achieved with the addition of 1.5% skim milk and 1.0×1011 cells/10 mL (152.07±0.78), whereas the values resulting from additions of 30% sucrose and 0.01% CaCl₂ treatments were 124.33±0.03 and 142.10±0.87, respectively. This implies that those values are the maximum values achievable for dextran production in L. mesenteroides CS-5. Thus, increasing the number of cells inoculated can facilitate optimum dextran production.
#Chonggak kimchi #Dextran #Leuconostoc mesenteroides #Viscosity

목차

Abstract
Introduction
Materials and Methods
Results and Discussion
References

참고문헌 (31)

참고문헌 신청
[학술지(정기간행물)] - Chung MJ / 1997 / Industrial application of lactic acid bacteria / Bioindustry News 10 (2) : 13 ~ 21 google schola [학술지(정기간행물)] - Jung HK / 2001 / Selection criteria for probiotics and their industrial applications / Bioindustry News 14 (1) : 39 ~ 48 google schola [학술지(정기간행물)] - Cha SK. / 2000 / Probiotic microorganisms and lactic acid bacteria foods / Micro-organisms and Industry 26 (2) : 13 ~ 21 google schola [학술지(정기간행물)] - Kwon OS / 2011 / Isolation and characterization of high dextran producer Leuconostoc mesenteroides 2A9 from cabbage kimchi / QBS 30 (2) : 147 ~ 152 google schola [단행본] - Holzapfel WH / 1992 / The prokaryotes, Vol 2 / Springer-Verlag : 1508 ~ 1534 google schola

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