지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.2
- 수록면
- 23 - 30 (8page)
이용수
초록· 키워드
오류제보하기
Purpose: Runx2 (runt-related transcription factor 2), a bone-specific transcription factor, is a key regulator of osteoblast differentiation and its expression is induced by the activation of BMP-2 signaling. This study examined whether zinc modulates BMP-2 signaling and therefore stimulates Runx2 and osteoblast differentiation gene expression. Methods: Two osteoblastic MC3T3-E1 cell lines (subclones 4 as a high osteoblast differentiation and subclone 24 as a low osteoblastic differentiation) were cultured in an osteogenic medium (OSM) as the normal control, Zn- (1 μM Zn) or Zn+ (15 μM Zn) for 24 h. The genes and proteins for BMP-2 signaling (BMP-2, Smad-1/p-Smad-1), transcription factors (Runx2, osterix), and osteoblast differentiation marker proteins were assessed. Results: In both cell lines, BMP-2 mRAN and protein expression and extracellular BMP-2 secretion all decreased in Zn-. The expression of Smad-1 (downstream regulator of BMP-2 signaling) and p-Smad-1 (phosphorylated Smad-1) also downregulated in Zn-. Furthermore, the expression of the bone-specific transcription factors, Runx2 and osterix, decreased in Zn-, which might be due to the decreased BMP-2 expression and Smad-1 activation (p-Smad-1) by Zn-, because Runx2 and osterix both are downstream in BMP-2 signaling. Bone marker gene expression, such as alkaline phosphatase (ALP), collagen type I (COLI), osteocalcin, and osteopontin were also downregulated in Zn-. Conclusion: The results suggest that a zinc deficiency in osteoblasts suppresses the BMP-2 signaling pathway via the suppression of Smad-1 activation, and this suppressed BMP-2 signaling can cause poor osteoblast differentiation.
목차
ABSTRACT
Introduction
Methods
Results
Discussion
Summary
References
참고문헌 (30)
참고문헌 신청
[학술지(정기간행물)] - Coleman JE / 1992 / Zinc proteins: enzymes, storage proteins, transcription factors, and replication proteins / Annu Rev Biochem 61 : 897 ~ 946
[학술지(정기간행물)] - Maret W / 2017 / Zinc in cellular regulation: the nature and significance of "Zinc Signals" / Int J Mol Sci 18 (11) : 2285 ~ 2296
[학술지(정기간행물)] - Tuerk MJ / 2009 / Zinc deficiency / Curr Opin Gastroenterol 25 (2) : 136 ~ 143
[학술지(정기간행물)] - Chabosseau P / 2016 / Zinc and diabetes / Arch Biochem Biophys 611 : 79 ~ 85
[학술지(정기간행물)] - Kim JH / 2014 / Regulation of the catabolic cascade in osteoarthritis by the Zinc-ZIP8-MTF1 Axis / Cell 156 (4) : 730 ~ 743
[학술지(정기간행물)] - Maret W / 2017 / Zinc in cellular regulation: the nature and significance of "Zinc Signals" / Int J Mol Sci 18 (11) : 2285 ~ 2296
[학술지(정기간행물)] - Tuerk MJ / 2009 / Zinc deficiency / Curr Opin Gastroenterol 25 (2) : 136 ~ 143
[학술지(정기간행물)] - Chabosseau P / 2016 / Zinc and diabetes / Arch Biochem Biophys 611 : 79 ~ 85
[학술지(정기간행물)] - Kim JH / 2014 / Regulation of the catabolic cascade in osteoarthritis by the Zinc-ZIP8-MTF1 Axis / Cell 156 (4) : 730 ~ 743
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
MC3T3-E1 세포에서 BMP2에 의한 조골세포의 분화에 일주기 유전자 Per1이 미치는 영향
생명과학회지
2017 .05
-
Zinc modulation of osterix in MC3T3-E1 cells
Journal of Nutrition and Health
2020 .08
-
Melanin extract from Gallus gallus domesticus promotes proliferation and differentiation of osteoblastic MG-63 cells via bone morphogenetic protein-2 signaling
Nutrition Research and Practice
2017 .06
-
Melanin extract from Gallus gallus domesticus promotes proliferation and differentiation of osteoblastic MG-63 cells via bone morphogenetic protein-2 signaling
Nutrition Research and Practice
2017 .06
-
Itch E3 Ubiquitin Ligase Positively Regulates TGF- Signaling to EMT via Smad7 Ubiquitination
Molecules and Cells
2015 .01
-
Rebalancing SMAD7/SMAD3 Signaling Reduces Adhesion Formation during Flexor Tendon Healing
Journal of Microbiology and Biotechnology
2023 .03
-
Molecular Mechanism of Runx2-Dependent Bone Development
Molecules and Cells
2020 .01
-
Aqueous extract of Petasites japonicus leaves promotes osteoblast differentiation via up-regulation of Runx2 and Osterix in MC3T3-E1 cells
Nutrition Research and Practice
2021 .10
-
Aqueous extract of Petasites japonicus leaves promotes osteoblast differentiation via up-regulation of Runx2 and Osterix in MC3T3-E1 cells
Nutrition Research and Practice
2021 .10
-
새만금 유역에서 논 최적관리기법의 수질개선 효과와 정책고려사항
한국습지학회지
2018 .01
-
Hederagenin의 뼈 형성 관련 작용 기전 연구
Journal of Nutrition and Health
2022 .12
-
Post-Translational Regulations of Transcriptional Activity of RUNX2
Molecules and Cells
2020 .01
-
Regulatory Role of Zinc in Immune Cell Signaling
Molecules and Cells
2021 .01
-
Transforming Growth Factor β1/Smad4 Signaling Affects Osteoclast Differentiation via Regulation of miR-155 Expression
Molecules and Cells
2017 .03
-
CROX (Cluster Regulation of RUNX) as a Potential Novel Therapeutic Approach
Molecules and Cells
2020 .01
-
Tectorigenin Promotes Osteoblast Differentiation and in vivo Bone Healing, but Suppresses Osteoclast Differentiation and in vivo Bone Resorption
Molecules and Cells
2018 .03
-
Longan (Dimocarpus longan Lour.) Fruit Extract Stimulates Osteoblast Differentiation via Erk1/2-Dependent RUNX2 Activation
Journal of Microbiology and Biotechnology
2016 .01
-
Role of RUNX Family Transcription Factors in DNA Damage Response
Molecules and Cells
2020 .01
-
SMAD4 Controls Cancer Cell Metabolism by Regulating Methylmalonic Aciduria Cobalamin Deficiency (cbl) B Type
Molecules and Cells
2022 .06
-
The Roles of RUNX Family Proteins in Development of Immune Cells
Molecules and Cells
2020 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2018-594-001807710