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논문 기본 정보

자료유형
학술저널
저자정보
Jiang Ke (Department of Orthopaedics Affiliated Hospital of North Sichuan Medical College Nanchong P.R. ChinaKey Laboratory of Emergency and Trauma Ministry of Education College of Emergency and Trauma Hainan M) Li Yuling (Department of Orthopaedics Affiliated Hospital of North Sichuan Medical College Nanchong P.R. China) Xiang Chao (Department of Orthopaedics Affiliated Hospital of North Sichuan Medical College Nanchong P.R. China) Xiong Yan (Department of Orthopaedics Daping Hospital Army Medical University Chongqing P.R. China) Jia Jiameng (Department of Rehabilitation Affiliated Hospital of North Sichuan Medical College Nanchong P.R. China)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제33권 제3호
발행연도
2023.3
수록면
339 - 347 (9page)
DOI
10.4014/jmb.2209.09033

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Transforming growth factor-β is a key factor in regulating adhesion formation during tendon healing. We investigated the effectiveness of SMAD family members, SMAD7 and SMAD3, in the TGFβ/Smad signaling during flexor tendon repair. Mouse flexor toe deep tendon rupture anastomosis models were made. On days 3, 7, 14, 21, and 28, the expressions of smad7 and smad3 in flexor tendon tissues were detected by RT-qPCR and western blot. Furthermore, postoperative intraperitoneal injections of SMAD7 agonists or SMAD3 antagonists were given. The degree of tendon healing was evaluated by adhesion testing and biomechanical experiments. Hematoxylin and eosin (HE) staining was used to observe the pathological changes. Immunohistochemistry was used to evaluate the expressions of collagen III, SMAD3, and SMAD7. The mRNA levels of matrix metalloproteinases, Mmp2 and Mmp9, and scleraxis (SCX) in flexor tendon tissue were detected by RT-qPCR. Smad3 expression increased and Smad7 expression decreased in flexor tendon tissue after injury. In addition, the SMAD7 agonist blocked SMAD3 phosphorylation. SMAD7 agonist and SMAD3 antagonist both improved adhesion formation during flexor tendon healing, and decreased the expressions of collagen III, Mmp9, and SCX, while increasing Mmp2 expression. This study provides a possible theoretical basis for the SMAD7-SMAD3 signal cascade during flexor tendon adhesion healing.

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