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Circadian Clock Gene Per1 Mediates BMP2-induced Osteoblast Differentiation in MC3T3-E1 Cells
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MC3T3-E1 세포에서 BMP2에 의한 조골세포의 분화에 일주기 유전자 Per1이 미치는 영향

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Type
Academic journal
Author
Hyeon-Young Min (대구대학교) Won-Gu Jang (대구대학교)
Journal
Korean Society Of Life Science Journal of Life Science Vol.27 No.5 KCI Accredited Journals
Published
2017.5
Pages
501 - 508 (8page)

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Circadian Clock Gene Per1 Mediates BMP2-induced Osteoblast Differentiation in MC3T3-E1 Cells
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Research history (4)

2020

Molecular Mechanism of Osteoblastic Differentiation and Smooth Muscle Cell Calcification by Circadian Clock Gene
민현영 생명공학과 2020.01 Thesis

2017

베타인(betaine)에 의한 조골세포의 분화 촉진효과
민현영 ,  장원구 한국식품영양과학회 학술대회발표집 2017.10 Proceeding
Circadian Clock Gene Per1 Mediates BMP2-induced Osteoblast Differentiation in MC3T3-E1 Cells
Hyeon-Young Min ,  Won-Gu Jang Journal of Life Science 2017.05 Academic journal

2015

Mechanism of Osteoblast Differentiation byBiological Rhythm Regulatory Gene
민현영 생명공학과 2015.01 Thesis
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Bone morphogenetic proteins (BMPs) are multifunctional cytokines that play important roles in a variety of cellular functions. Among BMP family members, BMP2 efficiently promotes osteoblast differentiation through Smad-mediated runt-related transcription factor 2 (Runx2) expression. Several recent studies suggest that BMPs are associated with clock genes, in particular Bmal1. Bmal1 protein heterodimerizes with Clock protein and then induces period 1 (Per1) expression. However, the role of Per1 on osteoblast differentiation remains unclear. In this study, we investigated whether Per1 is involved in osteoblast differentiation. MC3T3-E1 cells were treated with BMP2 for induction of osteoblastic differentiation. Osteogenic maker gene and Per1 mRNA expression were measured using real-time PCR. Interestingly, BMP2 treatment induced Per1 mRNA expression in MC3T3-E1 cells. To further investigate the function of Per1 on osteoblast differentiation, MC3T3-E1 cells were transiently transfected with pCMV-Per1. Per1 overexpression increased Runx2 mRNA and protein levels. Also, mRNA expression and promoter activity of osteocalcin were upregulated by Per1 overexpression. To investigate the effect of interaction between Per1 and osteogenic condition, MC3T3-E1 cells were cultured in osteogenic medium containing ascorbic acid and β-glycerophosphate. Osteogenic medium-induced ALP staining level and mineralization were synergistically increased by overexpression of Per1. Taken together, these results demonstrate that Per1 is a positive regulator of osteoblast differentiation.
#BMP2 #clock genes #osteoblasts #Per1 #Runx2

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References (38)

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Albrecht, U., Sun, Z. S., Eichele, G. and Lee, C. C. 1997. A differential response of two putative mammalian circadian regulators, mper1 and mper2, to light. Cell 91, 1055-1064. Bellows, C. G., Aubin, J. E. and Heersche, J. N. 1991. Initiation and progression of mineralization of bone nodules formed in vitro: the role of alkaline phosphatase and organic phosphate. Bone Miner. 14, 27-40. Bjarnason, G. A. and Jordan, R. 2000. Circadian variation of cell proliferation and cell cycle protein expression in man: clinical implications. Prog. Cell Cycle Res. 4, 193-206. Borba, V. Z. and Manas, N. C. 2010. The use of PTH in the treatment of osteoporosis. Arq. Bras. Endocrinol. Metabol. 54, 213-219. Dibner, C., Schibler, U. and Albrecht, U. 2010. The mammalian circadian timing system: organization and coordination of central and peripheral clocks. Annu. Rev. Physiol. 72, 517- 549.

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UCI(KEPA) : I410-ECN-0101-2018-470-000932971