지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.12
- 수록면
- 623 - 630 (8page)
이용수
초록· 키워드
오류제보하기
Plasmodium lactate dehydrogenase (pLDH) is a strong target antigen for the determination of infection with Plasmodium species specifically. However, a more effective antibody is needed because of the low sensitivity of the current antibody in many immunological diagnostic assays. In this study, recombinant Plasmodium vivax LDH (PvLDH) was experimentally constructed and expressed as a native antigen to develop an effective P. vivax-specific monoclonal antibody (mAb). Two mAbs (2CF5 and 1G10) were tested using ELISA and immunofluorescence assays (IFA), as both demonstrated reactivity against pLDH antigen. Of the 2 antibodies, 2CF5 was not able to detect P. falciparum, suggesting that it might possess P. vivax-specificity. The detection limit for a pair of 2 mAbs-linked sandwich ELISA was 31.3 ng/ml of the recombinant antigen. The P. vivax-specific performance of mAbs-linked ELISA was confirmed by in vitro-cultured P. falciparum and P. vivax-infected patient blood samples. In conclusion, the 2 new antibodies possessed the potential to detect P. vivax and will be useful in immunoassay.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (20)
참고문헌 신청
[인터넷자원] - Centers for Disease Control and Prevention / / Malaria
[학술지(정기간행물)] - Bartoloni A / 2012 / Clinical aspects of uncomplicated and severe malaria / Mediterr J Hematol Infect Dis 4 : e2012026 ~
[학술지(정기간행물)] - Baird JK / 2016 / Diagnosis and treatment of Plasmodium vivax malaria / Am J Trop Med Hyg 95 : 35 ~ 51
[학술지(정기간행물)] - Verma P / 2013 / Detection of histidine rich protein and lactate dehydrogenase of Plasmodium falciparum in malaria patients by sandwich ELISA using in-house reagents / Indian J Med Res 138 : 977 ~ 987
[학술지(정기간행물)] - Dzakah EE / 2013 / Plasmodium vivax aldolase-specific monoclonal antibodies and its application in clinical diagnosis of malaria infections in China / Malar J 12 : 199 ~
[학술지(정기간행물)] - Bartoloni A / 2012 / Clinical aspects of uncomplicated and severe malaria / Mediterr J Hematol Infect Dis 4 : e2012026 ~
[학술지(정기간행물)] - Baird JK / 2016 / Diagnosis and treatment of Plasmodium vivax malaria / Am J Trop Med Hyg 95 : 35 ~ 51
[학술지(정기간행물)] - Verma P / 2013 / Detection of histidine rich protein and lactate dehydrogenase of Plasmodium falciparum in malaria patients by sandwich ELISA using in-house reagents / Indian J Med Res 138 : 977 ~ 987
[학술지(정기간행물)] - Dzakah EE / 2013 / Plasmodium vivax aldolase-specific monoclonal antibodies and its application in clinical diagnosis of malaria infections in China / Malar J 12 : 199 ~
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UCI(KEPA) : I410-ECN-0101-2018-513-001680427