Microbial conversion of major ginsenosides in ginseng total saponins by Platycodon grandiflorum endophytes

Lei Cui; Song-quan Wu; Cheng-ai Zhao; Cheng-ri Yin

Microbial conversion of major ginsenosides in ginseng total saponins by Platycodon grandiflorum endophytes

Recommendations

Search

Questions

Type: Academic journal

Author: Lei Cui (Yanbian University) Song-quan Wu (Yanbian University) Cheng-ai Zhao (Jilin Agricultural University) Cheng-ri Yin (Yanbian University)

Journal: The Korean Society of Ginseng Journal of Ginseng Research Vol.40 No.4 KCI Candidated Journals SCIE

Published: 2016.10

Pages: 366 - 374 (9page)

Usage

📌

Topic

📖

Background

🔬

Method

🏆

Result

Microbial conversion of major ginsenosides in ginseng total saponins by Platycodon grandiflorum endophytes

Abstract· Keywords

Report Errors

Background: In this study, we screened and identified an endophyte JG09 having strong biocatalytic activity for ginsenosides from Platycodon grandiflorum, converted ginseng total saponins and ginsenoside monomers, determined the source of minor ginsenosides and the transformation pathways, and calculated the maximum production of minor ginsenosides for the conversion of ginsenoside Rb1 to assess the transformation activity of endophyte JG09.
Methods: The transformation of ginseng total saponins and ginsenoside monomers Rb1, Rb2, Rc, Rd, Rg1 into minor ginsenosides F2, C-K and Rh1 using endophyte JG09 isolated by an organizational separation method and Esculin-R2A agar assay, as well as the identification of transformed products via TLC and HPLC, were evaluated. Endophyte JG09 was identified through DNA sequencing and phylogenetic analysis.
Results: A total of 32 β-glucosidase-producing endophytes were screened out among the isolated 69 endophytes from P. grandiflorum. An endophyte bacteria JG09 identified as Luteibacter sp. effectively converted protopanaxadiol-type ginsenosides Rb1, Rb2, Rc, Rd into minor ginsenosides F2 and C-K, and converted protopanaxatriol-type ginsenoside Rg1 into minor ginsenoside Rh1. The transformation pathways of major ginsenosides by endophyte JG09 were as follows: Rb1→Rd→F2→C-K; Rb2→C-O→C-Y→C-K; Rc→C-Mc1→C-Mc→C-K; Rg1→Rh1. The maximum production rate of ginsenosides F2 and C-K reached 94.53% and 66.34%, respectively.
Conclusion: This is the first report about conversion of major ginsenosides into minor ginsenosides by fermentation with P. grandiflorum endophytes. The results of the study indicate endophyte JG09 would be a potential microbial source for obtaining minor ginsenosides.

#conversion #endophytes #minor ginsenosides #Platycodon grandiflorum

ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References

References (33)

Add References

[학술지(정기간행물)] - Qi LW / 2011 / Isolation and analysis of ginseng: advances and challenges / Nat Prod Rep 28 : 467 ~ 495

[학술지(정기간행물)] - Jia L / 2009 / Current evaluation of the millennium phytomedicine-ginseng (I):etymology, pharmacognosy, phytochemistry, market and regulations / Curr Med Chem 16 : 2475 ~ 2484

[학술지(정기간행물)] - Wang L / 2011 / Bioconversion of ginsenosides Rb1, Rb2, Rc and Rd by novel b-glucosidase hydrolyzing outer 3-O glycoside from Sphingomonas sp. 2F2: Cloning, expression, and enzyme characterization / J Biotechnol 156 : 125 ~ 133

[학술지(정기간행물)] - Li W / 2011 / Snailase preparation of ginsenoside M1 from protopanaxadiol-type ginsenoside and their protective effects against CCl4-induced chronic hepatotoxicity in mice / Molecules 16 : 10093 ~ 10103

[학술지(정기간행물)] - Chen Y / 2013 / Anti-cancer effects of ginsenoside compound k on pediatric acute myeloid leukemia cells / Cancer Cell Int 13 : 24 ~

Recommendations

It is an article recommended by DBpia according to the article similarity. Check out the related articles!

Related Authors

Lei Cui

Affiliation Yanbian University

Fields Medicine and Pharmacy > Other Medicine and Pharmacy

Articles 1 Usage 12

Song-quan Wu

Affiliation Yanbian University

Fields Medicine and Pharmacy > Other Medicine and Pharmacy

Articles 1 Usage 12

Cheng-ai Zhao

Affiliation Jilin Agricultural University

Fields Medicine and Pharmacy > Other Medicine and Pharmacy

Articles 1 Usage 12

Cheng-ri Yin

Affiliation Yanbian University

Fields Medicine and Pharmacy > Other Medicine and Pharmacy

Articles 1 Usage 12

Frequently Viewed Together

Enzymatic Transformation of Ginsenoside Rb1 by Lactobacillus pentosus Strain 6105 from Kimchi

Se-Hwa Kim , Jin-Woo Min , Lin-Hu Quan and others 3 Journal of Ginseng Research 2012 .07

Comments(0)

Write first comments.

UCI(KEPA) : I410-ECN-0101-2018-524-001598496

구분	그룹	데이터 항목
AI 학습용 데이터	원문	원문 PDF 파일
AI 학습용 데이터	원문 + 메타 (기본/상세)	원문 PDF 파일 및 서지정보 CSV
대량 구매용 데이터	B2B 구독 방식	특정 자료 한정으로 원문 접근 권한 부여
대량 구매용 데이터	URL 전달 방식	바로 PDF 뷰어를 열람할 수 있는 URL 제공

구분	그룹	데이터 항목
AI 학습용 데이터	기본 메타	발행기관명, 간행물명, 권호명, 권(vol), 호(issue), 통권, 발행연도, 발행월, 논문명, 저자명, 시작페이지, 종료페이지, 전체페이지, 상세페이지URL
상세 메타 데이터	발행기관 메타	발행기관 이명, 영문명, 창립연도, 홈페이지URL, 발행기관 소개
	간행물 메타	부제목, 간행물 유형, ISSN, ISBN, 최초발행연도, 폐간연도, 간행빈도, 발행주기, 등재사항, 이용수, 피인용수, 권호수, 논문수, 표지이미지
	논문 메타	작성 언어, 부제목, 대등제목, 목차, 키워드, 초록, 이미지, 참고문헌, 이용수, 피인용수, 논문활용도, DBpia통합주제분류, KDC분류, DDC분류, 한국연구재단분류, UCI, DOI
	저자 메타	소속기관, 소속부서, 직급, 연구분야, 연구키워드, 이용수, 피인용수, 저자 논문활용도

구분	그룹	데이터 항목
※ 결합형/맞춤형 메타 데이터는 신청 내용에 따라 다양하게 제공 가능
이용순위 정보	주제분야별 많이 이용된 논문	“인문학”에서 많이 이용된 논문 TOP100
	이용기관별 많이 이용된 논문	“중고등학교”에서 많이 이용된 논문 TOP100
	세부기관별 많이 이용된 논문	“서울대학교”에서 많이 이용된 논문 TOP100
	키워드별 많이 이용된 논문	“Chat GPT”에서 많이 이용된 논문 TOP100
키워드 정보	많이 이용된 키워드	특정기간/분야/저널 내 많이 이용된 키워드
	많이 발행된 키워드	특정기간/분야/저널 내 많이 발행된 키워드
	많이 검색된 키워드	특정기간/분야/저널 내 많이 검색된 키워드
	연구 트렌드 키워드	특정 키워드 연관 연구동향 분석 데이터 키워드

논문 기본 정보

Abstract· Keywords

AI Summary

Topic

Background

Method

Result

주요내용

Contents

References (33)

Recommendations

Related Authors

Frequently Viewed Together

Recently viewed articles

Comments(0)