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논문 기본 정보

자료유형
학술저널
저자정보
Hua Yao (Chinae-Japan Union Hospital of Jilin University) Xuwen Li (Jilin University) Ying Liu (Jilin University) Qian Wu (Jilin University) Yongri Jin (Jilin University)
저널정보
고려인삼학회 Journal of Ginseng Research Journal of Ginseng Research Vol.40 No.4
발행연도
2016.10
수록면
415 - 422 (8page)

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초록· 키워드

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Background: Rare ginsenosides in Panax quinquefolius L. have strong bioactivities. The fact that it is hard to obtain large amounts of rare ginsenosides seriously restricts further research on these compounds. An easy, fast, and efficient method to obtain different kinds of rare ginsenosides simultaneously and to quantify each one precisely is urgently needed.
Methods: Microwave-assisted extraction (MAE) was used to extract nine kinds of rare ginsenosides from P. quinquefolius L. In this article, rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5] were identified by high performance liquid chromatography (HPLC)eelectrospray ionization emass spectrometry. The quantity information of rare ginsenosides was analyzed by HPLC-UV at 203 nm.
Results: The optimal conditions for MAE were using water as solvent with the material ratio of 1:40 (w/v) at a temperature of 145C, and extracting for 15 min under microwave power of 1,600 W. Seven kinds of rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, Rk1, and Rg5] had high extraction yields, but those of 20(S)-Rg3 and 20(R)-Rg3 were lower. Compared with the conventional method, the extraction yields of the nine rare ginsenosides were significantly increased.
Conclusion: The results indicate that rare ginsenosides can be extracted effectively by MAE from P. quinquefolius L. in a short time. Microwave radiation plays an important role in MAE. The probable generation process of rare ginsenosides is also discussed in the article. It will be meaningful for further investigation or application of rare ginsenosides.

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ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References

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