지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.8
- 수록면
- 385 - 391 (7page)
이용수
초록· 키워드
오류제보하기
The discovery and understanding of antigenic proteins are essential for development of a vaccine against malaria. In Plasmodium falciparum, Pf92 have been characterized as a merozoite surface protein, and this protein is expressed at the late schizont stage, but no study of Pv92, the orthologue of Pf92 in P. vivax, has been reported. Thus, the protein structure of Pv92 was analyzed, and the gene sequence was aligned with that of other Plasmodium spp. using bioinformatics tools. The recombinant Pv92 protein was expressed and purified using bacterial expression system and used for immunization of mice to gain the polyclonal antibody and for evaluation of antigenicity by protein array. Also, the antibody against Pv92 was used for subcellular analysis by immunofluorescence assay. The Pv92 protein has a signal peptide and a sexual stage s48/45 domain, and the cysteine residues at the N-terminal of Pv92 were completely conserved. The N-terminal of Pv92 was successfully expressed as soluble form using a bacterial expression system. The antibody raised against Pv92 recognized the parasites and completely merged with PvMSP1-19, indicating that Pv92 was localized on the merozoite surface. Evaluation of the human humoral immune response to Pv92 indicated moderate antigenicity, with 65% sensitivity and 95% specificity by protein array. Taken together, the merozoite surface localization and antigenicity of Pv92 implicate that it might be involved in attachment and invasion of a merozoite to a new host cell or immune evasion during invasion process.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (27)
참고문헌 신청
[보고서] - WHO / 2015 / World Malaria Report (2015)
[학술지(정기간행물)] - Mueller I / 2015 / Development of vaccines for Plasmodium vivax malaria / Vaccine 33 : 7489 ~ 7495
[학술지(정기간행물)] - Herrera S / 2007 / An update on the search for a Plasmodium vivax vaccine / Trends Parasitol 23 : 122 ~ 128
[학술지(정기간행물)] - Chen JH / 2010 / Immunoproteomics profiling of blood stage Plasmodium vivax infection by high-throughput screening assays / J Proteome Res 9 : 6479 ~ 6489
[학술지(정기간행물)] - Arevalo-Pinzon G / 2010 / Conserved high activity binding peptides from the Plasmodium falciparum Pf34 rhoptry protein inhibit merozoites in vitro invasion of red blood cells / Peptides 31 : 1987 ~ 1994
[학술지(정기간행물)] - Mueller I / 2015 / Development of vaccines for Plasmodium vivax malaria / Vaccine 33 : 7489 ~ 7495
[학술지(정기간행물)] - Herrera S / 2007 / An update on the search for a Plasmodium vivax vaccine / Trends Parasitol 23 : 122 ~ 128
[학술지(정기간행물)] - Chen JH / 2010 / Immunoproteomics profiling of blood stage Plasmodium vivax infection by high-throughput screening assays / J Proteome Res 9 : 6479 ~ 6489
[학술지(정기간행물)] - Arevalo-Pinzon G / 2010 / Conserved high activity binding peptides from the Plasmodium falciparum Pf34 rhoptry protein inhibit merozoites in vitro invasion of red blood cells / Peptides 31 : 1987 ~ 1994
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UCI(KEPA) : I410-ECN-0101-2017-513-001124475