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자료유형
학술저널
저자정보
Chae Jeong Lim (Seoul National University) Yeo Sung Yoon (Seoul National University) Pan Dong Ryu (Seoul National University)
저널정보
대한약침학회 Journal of Acupuncture & Meridian Studies Journal of Acupuncture & Meridian Studies Vol.13 No.1
발행연도
2020.1
수록면
33 - 38 (6page)

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The primo vascular system (PVS) is reported to have a periductium composed of cells with spherical or spindle-shaped nuclei and abundant cytoplasm. However, little is known about these periductium cells. In this study, we examined the morphological features of cells covering the PVS tissue isolated from the surface of abdominal organs of rats. By hematoxylin and eosin (H&E) staining, we observed a layer of dark nuclei on the basement membrane at the borders of the sections of primo node (PN), primo vessel (PV), and their subunits. The nuclei appeared thin and linear (10-14 mm), elliptical (8-10 Â 3-4 mm), and round (5-7 mm). The borders of the PVS tissue sections were immunostained with a selective antibody for mesothelial cells (MCs). Areas of immunoreactivity overlapped with the flattened cells are shown by hematoxylin and eosin staining. By scanning electron microscopy, we further identified elliptical (11 Â 21 mm) and rectangular squamous MCs (length, 10 mm). There were numerous stomata (w200 nm) and microparticles (20-200 nm) on the surface of the PVS MCs. In conclusion, this study presents the novel finding that the PVS periductium is composed of squamous MCs. These cells tightly line the luminal surface of the PVS tissue, including PNs, PVs, and small branches of the PVs in the abdominal cavity. These results will help us to understand the physiological roles such as hyaluronan secretion and the fine structure of PVS tissue.

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