Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection

Mu-Xin Chen; Jia-Xu Chen; Shao-Hong Chen; Da-Na Huang; Lin Ai; Ren-Li Zhang

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자료유형: 학술저널

저자정보: Mu-Xin Chen (National Health and Family Planning Commission) Jia-Xu Chen (National Health and Family Planning Commission) Shao-Hong Chen (National Health and Family Planning Commission) Da-Na Huang (Shenzhen Center for Diseases Control and Prevention) Lin Ai (National Health and Family Planning Commission) Ren-Li Zhang (Shenzhen Center for Diseases Control and Prevention)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.54 No.3

발행연도: 2016.6

수록면: 375 - 382 (8page)

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Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.

#Angiostrongylus cantonensis #lateral flow immunoassay #monoclonal antibody #antigen detection

참고문헌 (37)

참고문헌 신청

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[학술지(정기간행물)] - Eamsobhana P / 2009 / Immunological diagnosis of human angiostrongyliasis due to Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) / Int J Infect Dis 13 : 425 ~ 431

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