Neuronal Apoptosis : Pathological Basis of Behavioral Dysfunctions Induced by Angiostrongylus cantonensis in Rodents Model :Pathological Basis of Behavioral Dysfunctions Induced by Angiostrongylus cantonensis in Rodents Model

Shiqi Luo; Lisi OuYang; Jie Wei; Feng Wu; Zhongdao Wu; Wanlong Lei; Dongjuan Yuan

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자료유형: 학술저널

저자정보: Shiqi Luo (Sun Yat-sen University) Lisi OuYang (Sun Yat-sen University) Jie Wei (The Second Affiliated Hospital of Guangzhou Medical University) Feng Wu (Sun Yat-sen University) Zhongdao Wu (Sun Yat-sen University) Wanlong Lei (Sun Yat-sen University) Dongjuan Yuan (Sun Yat-sen University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.55 No.3

발행연도: 2017.6

수록면: 267 - 285 (19page)

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Angiostrongylus cantonensis invades the central nervous system (CNS) of humans to induce eosinophilic men¬ingitis and meningoencephalitis and leads to persistent headache, cognitive dysfunction, and ataxic gait. Infected mice (nonpermissive host), admittedly, suffer more serious pathological injuries than rats (permissive host). However, the patho¬logical basis of these manifestations is incompletely elucidated. In this study, the behavioral test, histological and immu¬nohistochemical techniques, and analysis of apoptotic gene expression, especially caspase-3, were conducted. The movement and motor coordination were investigated at week 2 post infection (PI) and week 3 PI in mice and rats, respec¬tively. The cognitive impairs could be found in mice at week 2 PI but not in rats. The plaque-like lesion, perivascular cuffing of inflammatory cells, and dilated vessels within the cerebral cortex and hippocampus were more serious in mice than in rats at week 3 PI. Transcriptomic analysis showed activated extrinsic apoptotic pathway through increased ex¬pression of TNFR1 and caspase-8 in mice CNS. Immunohistochemical and double-labeling for NeuN and caspase-3 in¬dicated the dramatically increased expression of caspase-3 in neuron of the cerebral cortex and hippocampus in mice but not in rats. Furthermore, western-blotting results showed high expression of cleaved caspase-3 proteins in mice but relatively low expression in rats. Thus, extrinsic apoptotic pathway participated in neuronal apoptosis might be the patho¬logical basis of distinct behavioral dysfunctions in rodents with A. cantonensis infection. It provides the evidences of a pri¬mary molecular mechanism for the behavioral dysfunction and paves the ways to clinical diagnosis and therapy for A. cantonensis infection.

#Angiostrongylus cantonensis #eosinophilic meningitis #meningoencephalitis #apoptosis

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