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자료유형
학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제17권 제1호
발행연도
1987.6
수록면
15 - 21 (7page)

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The production of neutralizing antibodies against Hantaan and Seoul viruses in sera of five patients diagnosed as typical Korean hemorrhagic fever was investigated using plaque reduction neutralization test. Cross reactivity of sera of the patients to Hantaan and Seoul viruses which are related but distinct antigenically was also studied. The patients with Korean hemorrhagic fever have been bled at weekly intervals from 3 days to 66 days after the onset of illness and sera were prepared and stored in -65C until use. Neutralizing antibody titers against Hantaan and Seoul viruses were determined by a serum dilution plaque reduction neutralization (PRN) method employing immunoperoxidase staining in Vero E6 cells. In gerneral, the antibodies were detected as early as 3 days after the onset of disease. The peak titers were reached at around 2 weeks after the onset of illness and the antibodies were persisted up to 66 days although the titers were decreased slowly. All sera used from the patients were neutralized Hantaan viruses in higher titers in contrast with lower titers with Seoul viruses. It can be concluded that HFRS patients in this study were infected with Hantaan virus in rural areas of Korea.

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