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학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제24권 제2호
발행연도
1994.12
수록면
161 - 165 (5page)

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Hantaan virus, a causative agent of Korean hemorrhagic fever, now called hemorrhagic fever with renal syndrome was identified in 1976, resisted on Arbovirus Catalogue in 1978 and classified as a species of genus Hantavirus, Family Bunyaviridae. Hantaan virus has lipid-containing envelope and easilly loses its infectivity by treatment with lipid solvents such as acetone, xylene, ether, and can be inactivated at 37C for 48 hrs, and 56C 30 min. So Hantaan virus suspension and infected tissue have been kept at -70C for long-term storage. In this study, Hantaan virus suspension in sucrose and was treated with acetone, dried, and powered in vacuum. Powdered Hantaan virus was kept at room temperature for 4-6 months and then suspended in original volume of distilled water. This Hantaan virus suspension was inoculated into ICR suckling mouse brains, paralysed mice were killed, brains were collected and non-paralysed mice were killed on day-19 after inoculation of virus suspension. Collected brains were stained and examined by ordinary indirect fluorescent antibody technic. Hantaan virus antigens were found in brains inoculated with 4 month-exposed virus but brains inoculated with 5 month-kept virus showed Hantaan virus antigen only in 1 brsin out of 13. Brains inoculated with the 6 month-exposed at room temperature showed no specific fluorescence. So, infectivity of Hantaan virus could be preserved at least for 4 months at room temperature by sucrose-acetone treatment.

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