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자료유형
학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제15권 제1호
발행연도
1985.6
수록면
73 - 79 (7page)

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After primary reaction of Hantaan virus antigen and antiserum, different FITC-conjugated anti-IgG were applied to antigen-antibody complex for observation of cross reactivity between different origin of IgG. Immune sera preparated by immunizing S.D. rats, Balb/c mice and rabbits with Hantaan virus and human convalescent serum from Hemorrhagic fever with renal syndrome (HFRS) patients were used to test the cross reaction to different animal FITC-conjugated anti-immunoglobulin G, using the indirect immuno- fluorescent antibody different (IFA) technique. The results were as follows: 1. Human convalescent serum from HFRS patient which was combined with Hantaan virus antigen cross reacted to eight FITC-conjugated animal anti-immunoglobulin G sera. 2. Rat immune serum which was combined with Hantaan virus antigen did not cross react to FITC- goat anti-rabbit IgG for 1 hour reaction, but for 4 hour reaction it cross reacted to FITC-goat anti- rabbit IgG serum. 3. Mouse immune serum which was combined with Hantaan virus antigen did not cross react to FITC- goat anti-rahbit IgG. 4. Rabbit immune serum which was combined with Hantaan virus antigen did not cross react to FITC rabbit anti-human IgG, anti-rat IgG and anti-mouse IgG.

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