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논문 기본 정보

자료유형
학술저널
저자정보
Keyong Ho Lee (코오롱생명과학) Won Ho Yoon (서일대학)
저널정보
한국식품과학회 Food Science and Biotechnology Food Science and Biotechnology vol 18. no 5
발행연도
2009.10
수록면
1,253 - 1,257 (5page)

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Exo-polysaccharide isolated from the culture of Grifola frondosa was modified by sodium periodate (NaIO₄) and sodium chlorite (NaClO₂) to delete polysaccharide part and phenolic compound, respectively, and was investigated what effect has each part of exo-polysaccharide against 2,2’-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress in porcine kidney epithelial cells (LLC-PK1). Oxidative stress on LLC-PK1 cell was measured by cell viability, lipid peroxidation, superoxide dismutase (SOD), and glutathione peroxidase (GSH-px) activity. Exposure of LLC-PK1 cells to 1 mM AAPH for 24 hr resulted in significant decrease in cell viability, SOD, and GSH-px action, and significant increase in lipid peroxidation. The treatment of exo-polysaccharide and NaIO₄ modified sample protected LLC-PK1 cells from AAPHinduced cell damage such as cell viability, lipid peroxidation, SOD, and GSH-px activity in a dose dependant manner (10, 100, and 500 ㎍/㎖). However, the treatment of NaClO₂ modified sample did not affect for cell viability, lipid peroxidation, SOD, and GSH-px activity. The antioxidant activity of exo-polysaccharide was significantly decreased on AAPH-induced LLC-PK1 cell system when phenolic compound was deleted. The antioxidant activity was significantly correlated with the content of phenolic compound of exo-polysaccharide.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
References

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