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논문 기본 정보

자료유형
학술저널
저자정보
Sin-Hee Han (국립원예특작과학원) Young-Guk Kim (국립원예특작과학원) Su-Hwan Lee (국립원예특작과학원) Chung-Berm Park (국립원예특작과학원) Han-Gil Choi (원광대학교) Hye-Jin Jang (원광대학교) Young-Seob Lee (원광대학교) Dong-Yeul Kwon (원광대학교)
저널정보
한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.15 No.3
발행연도
2010.9
수록면
206 - 212 (7page)

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초록· 키워드

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Codium fragile (CF) is an edible green alga consumed as a traditional food source in Korea. In this study, the ethanol extract of CF was evaluated to determine if it has anti-inflammatory activity. Lipopolysaccharide (LPS), a toxin from bacteria, is a potent inducer of inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6. Therefore, we studied whether CF extracts have an anti-inflammatory effect in LPS-induced murine macrophage cell lines (RAW 264.7). In the present study, IL-6 production was measured using an enzymelinked immunosorbent assay (ELISA), prostaglandin E₂ (PGE₂) production was measured using the EIA kit, and cyclooxygenase (COX)-2 and mitogen-activated protein kinase (MAPK) activation were determined by Western blot analysis. IL-6 mRNA, COX-2 mRNA and iNOS mRNA expression were measured using reverse transcriptionpolymerase chain reaction (RT-PCR). The results indicated that CF extracts inhibit LPS-induced IL-6, NO and PGE₂ production in a dose-dependent manner, as well as expression of iNOS and COX-2. CF extracts significantly inhibited LPS-induced c-Jun N-terminal kinase (JNK) 1/2 phosphorylation. Taken together, these findings may help elucidate the mechanism by which CF modulates RAW 264.7 cell activation under inflammatory conditions.

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Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

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UCI(KEPA) : I410-ECN-0101-2012-511-003686998