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논문 기본 정보

자료유형
학술저널
저자정보
In-Ohk Ouh (Division of Vaccine Development Coordination, Center for Vaccine Research National Institute of Infectious Diseases, Korea National Institute of Health, Korea Disease Control and Prevention) Min Jung Kim (College of Veterinary, Gyeongsang National University) Kwang wook Kim (Division of Vaccine Development Coordination, Center for Vaccine Research National Institute of Infectious Diseases, Korea National Institute of Health, Korea Disease Control and Prevention) Hee ji Lim (Division of Vaccine Development Coordination, Center for Vaccine Research National Institute of Infectious Diseases, Korea National Institute of Health, Korea Disease Control and Prevention) Ye Jin Yang (College of Veterinary, Gyeongsang National University) Hu-Jang Lee (College of Veterinary, Gyeongsang National University) Woo H. Kim (College of Veterinary, Gyeongsang National University) Phil-Ok Koh (College of Veterinary, Gyeongsang National University) Seo Young Moon (Division of Vaccine Development Coordination, Center for Vaccine Research National Institute of Infectious Diseases, Korea National Institute of Health, Korea Disease Control and Prevention) Kyoung Hwa Choi (Division of Vaccine Development Coordination, Center for Vaccine Research National Institute of Infectious Diseases, Korea National Institute of Health, Korea Disease Control and Prevention) Yoo-Kyoung Lee (Division of Vaccine Development Coordination, Center for Vaccine Research National Institute of Infectious Diseases, Korea National Institute of Health, Korea Disease Control and Prevention) Kwang Il Park (College of Veterinary, Gyeongsang National University)
저널정보
한국예방수의학회 예방수의학회지 Journal of Preventive Veterinary Medicine Vol.48 No.3
발행연도
2024.9
수록면
158 - 167 (10page)

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This study evaluated the immunogenicity of the Bacillus Calmette-Guérin (BCG) vaccine in a guinea pig model to refine preclinical assessment methods. 24 guinea pigs were divided into four groups for immunohistochemical, histopathological, and molecular analyses, including qRT-PCR and ELISA. The ELISA results revealed significant elevations in interleukin 2 (IL-2), interferon-gamma (IFN- ), and tuberculosis-specific antibodies in vaccinated guinea pigs, particularly γ notable after 6 weeks. Although lung cytokine levels remained unchanged, spleen gene expression showed significant differences in interleukin-17, interleukin-12, interleukin-1β, and C-X-C motif chemokine ligand 10 after 6 weeks. Immunohistochemistry revealed peak IL-2 expression at 8 weeks and significant IFN-γ and TNF-α expression at 6 weeks. This study confirmed the effectiveness of BCG vaccine in guinea pigs, providing crucial insights for future tuberculosis vaccine development and standardizing immune response indicators.

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