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논문 기본 정보

자료유형
학술저널
저자정보
김진주 (연세대학교) 김혜민 (연세의대 병리과) 김홍경 (중앙대광명병원) 김수정 (연세대학교) 최종락 (연세대학교) 신새암 (연세대학교) 황도유 (연세대학교 의과대학)
저널정보
대한암학회 Cancer Research and Treatment Cancer Research and Treatment Vol.56 No.1
발행연도
2024.1
수록면
314 - 323 (10page)
DOI
10.4143/crt.2023.667

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Purpose We designed and evaluated the clinical performance of a plasma circulating tumor DNA (ctDNA) panel of 112 genes in various subtypes of lymphoma.Materials and Methods Targeted deep sequencing with an error-corrected algorithm was performed in ctDNA from plasma samples that were collected before treatment in 42 lymphoma patients. Blood buffy coat was utilized as a germline control. We evaluated the targeted gene panel using mutation detection concordance on the plasma samples with matched tissue samples analyzed the mutation profiles of the ctDNA.Results Next-generation sequencing analysis using matched tissue samples was available for 18 of the 42 patients. At least one mutation was detected in the majority of matched tissue biopsy samples (88.9%) and plasma samples (83.3%). A considerable number of mutations (40.4%) that were detected in the tissue samples were also found in the matched plasma samples. Majority of patients (21/42) were diffuse large B cell lymphoma patients. The overall detection rate of ctDNA in patients was 85.7% (36/42). The frequently mutated genes included <i>PIM1, TET2, BCL2, KMT2D, KLHL6, HIST1H1E</i>, and <i>IRF8</i>. A cutoff concentration (4,506 pg/mL) of ctDNA provided 88.9% sensitivity and 82.1% specificity to predict ctDNA mutation detection. The ctDNA concentration correlated with elevated lactate dehydrogenase level and the disease stage.Conclusion Our design panel can detect many actionable gene mutations, including those at low frequency. Therefore, liquid biopsy can be applied clinically in the evaluation of lymphoma patients, especially in aggressive lymphoma patients.

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