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논문 기본 정보

자료유형
학술저널
저자정보
Choi Mi Kyung (Department of Agriculture and Industries, Kangwon National University, Chuncheon, Kangwon 24341, Republic of Korea) Azeez Bimpe Suliyat (Department of Agriculture and Industries, Kangwon National University, Chuncheon, Kangwon 24341, Republic of Korea) Lee Sang Woo (International Biological Material Research Center, KRIBB, Daejeon 34141, Republic of Korea) Li Wan Yi (Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650200, P.R. China) Choi Sangho (International Biological Material Research Center, KRIBB, Daejeon 34141, Republic of Korea) Choi Ik-Young (Department of Agriculture and Industries, Kangwon National University, Chuncheon, Kangwon 24341, Republic of KoreaDepartment of SmartFarm and Agricultural Industry, Kangwon National Universi) Choi Ki-Young (Department of Agriculture and Industries, Kangwon National University, Chuncheon, Kangwon 24341, Republic of KoreaDepartment of SmartFarm and Agricultural Industry, Kangwon National Universi) Na Jong-Kuk (Department of Agriculture and Industries, Kangwon National University, Chuncheon, Kangwon 24341, Republic of KoreaDepartment of SmartFarm and Agricultural Industry, Kangwon National Universi)
저널정보
한국식물생명공학회 Journal of Plant Biotechnology Journal of Plant Biotechnology 제51권 제1호
발행연도
2024.3
수록면
33 - 49 (17page)
DOI
10.5010/JPB.2024.51.004.033

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초록· 키워드

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Pistacia weinmannifolia has long been used as an herbal medicine for treating various illnesses. The genomic information of P. weinmannifolia will help elucidate the chemical constituents that exert medicinal effects; however, genomic studies have rarely been performed. Therefore, we conducted a transcriptome analysis of P. weinmannifolia using the Illumina RNA sequencing system. We obtained a total of 18 million high-quality paired-end reads with 2,230 Mbp. De novo assembly of high-quality reads generated a total of 18,956 non-redundant contigs with an average length of 901 bp, of which 18,296 contigs (96.5%) were annotated. The total length of all unigenes was 17,080,830 bp, and the GC content and N50 were 43.2% and 1,137 bp, respectively. Annotation using The Kyoto Encyclopedia of Genes and Genomes (KEGG) assigned a total of 5,095 unigenes (26.9%), of which 3,166 were mapped to 410 different KEGG meta- bolic pathways. A comparison of unigenes between P. weinmannifolia and Pistacia chinensis showed that 8,825 unigenes were highly similar to each other. Simple sequence repeats were mined, and valuable data for further comparative and functional genomic studies were obtained to uncover the mechanisms underlying the medicinal properties of P. weinmannifolia as an important medicinal plant. Several genes of P.weinmannifolia involved in the biosynthesis of eugenol and isoeugenol were also identified in this study.

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