In vitro rapid propagation technology system of Dendrobium moniliforme (L.) Sw., a threatened orchid species in China

Liu Xu; Sun Liyong; Nie Tangjie; Chen Yao; Yin Zengfang

doi:10.1007/s11816-023-00838-5

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자료유형: 학술저널

저자정보: Liu Xu (Co-Innovation Center for Sustainable Forestry in Southern China) Sun Liyong (Co-Innovation Center for Sustainable Forestry in Southern China) Nie Tangjie (Co-Innovation Center for Sustainable Forestry in Southern China) Chen Yao (Co-Innovation Center for Sustainable Forestry in Southern China) Yin Zengfang (Co-Innovation Center for Sustainable Forestry in Southern China)

저널정보: 한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제17권 제3호

발행연도: 2023.6

수록면: 369 - 378 (10page)

DOI: 10.1007/s11816-023-00838-5

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Dendrobium moniliforme is a threatened and medicinal orchid species in China. The establishment of in vitro rapid propa- gation technology system will facilitate preservation and utilization of germplasm resources in D. moniliforme. In this study, the effects of different plant growth regulators (PGRs) on seed germination, proliferation of cluster shoots, rooting of shoots were tested under asymbiotic culture condition, and the best acclimatization condition of plantlets was screened. Our research results were shown as follows: germination rate (GR) was significantly increased by adding N6-benzyladenine (BA, 0.1 mg/L) during asymbiotic culture. In Murashige and Skoog (MS) medium supplemented with α-naphthaleneacetic acid (NAA, 1.0 mg/L) and BA (1.0 mg/L), the proliferation coefficient of cluster shoots was 6.1 times comparing with the control group. The MS medium with NAA (0.5 mg/L) and indole-3-butyric acid (IBA, 0.5 mg/L) promoted the highest rooting rate (RR). For plantlet transplanting, the equal mixing volume ratio of pine bark, turfy soil, and peanut shells was the best substrate. In a word, this rapid propagation system provides strong technical support for D. moniliforme to expand proliferation and germplasm resource protection.

#Dendrobium moniliforme · Seed germination · Rapid propagation technology system · Plant growth regulators · Asymbiotic culture

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