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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 673 - 680 (8page)
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초록· 키워드
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The purpose of this study was to establish a basic principal procedure for the processing of cultured meat. The first stage involved isolating satellite cells from the desired muscle of an animal using enzymatic digestion (i.e., by using proteases, collagenases, and pronases). The second stage involved culturing the isolated muscle satellite cells in a growth medium containing fetal bovine serum and penicillin/streptomycin with growth factors for an optimal period of time. The second stage involved a basic method for the isolated muscle cells to proliferate while sub-culturing to further induce differentiation in gelatin-coated culture dishes with the general culture medium. The third stage involved the induction of differentiation of muscle satellite cells or formation of myotubes using myogenic medium. Lastly, the fourth stage involved the identification of cell differentiation or myotube formation (myogenesis) using fluorescent dyes. Moreover, the principle of these protocols can be applied to perform primary culture of animal cells. This study will assist beginners with the technical aspects of culturing meat (isolation, cultivation, and differentiation of muscle satellite cells as well as identification of myotube formation for myogenesis).
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등록된 정보가 없습니다.
참고문헌 (5)
참고문헌 신청
Hanna L. Tuomisto / 2011 / Environmental Impacts of Cultured Meat Production / Environmental Science & Technology 45(14):6117~6123
G. Owen Schaefer / 2014 / The ethics of producing in vitro meat / Journal of Applied Philosophy 31(2):188~202
Zuhaib F. Bhat / 2019 / Technological, Regulatory, and Ethical Aspects ofIn VitroMeat: A Future Slaughter‐Free Harvest / Comprehensive Reviews in Food Science and Food Safety 18(4):1192~1208
Mark Guthridge / 1992 / The Role of Basic Fibroblast Growth Factor in Skeletal Muscle Regeneration / Growth Factors 6(1):53~63
Ronald E. Allen / 1989 / Regulation of skeletal muscle satellite cell proliferation and differentiation by transforming growth factor-beta, insulin-like growth factor I, and fibroblast growth factor / Journal of Cellular Physiology 138(2):311~315
G. Owen Schaefer / 2014 / The ethics of producing in vitro meat / Journal of Applied Philosophy 31(2):188~202
Zuhaib F. Bhat / 2019 / Technological, Regulatory, and Ethical Aspects ofIn VitroMeat: A Future Slaughter‐Free Harvest / Comprehensive Reviews in Food Science and Food Safety 18(4):1192~1208
Mark Guthridge / 1992 / The Role of Basic Fibroblast Growth Factor in Skeletal Muscle Regeneration / Growth Factors 6(1):53~63
Ronald E. Allen / 1989 / Regulation of skeletal muscle satellite cell proliferation and differentiation by transforming growth factor-beta, insulin-like growth factor I, and fibroblast growth factor / Journal of Cellular Physiology 138(2):311~315
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