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논문 기본 정보

자료유형
학술저널
저자정보
하지용 (계명대학교) Lee Eun Hye (Joint Institute for Regenerative Medicine Kyungpook National University) Chun So Young (BioMedical Research Institute Kyungpook National University Hospital Daegu Korea.) Lee Jun Nyung (Department of Urology School of Medicine Kyungpook National University Daegu Korea.Department of Urology Kyungpook National University Chilgok Hospital Daegu Korea.) Ha Yun-Sok (Department of Urology Kyungpook National University Hospital Daegu Korea.Department of Urology School of Medicine Kyungpook National University Daegu Korea.) Chung Jae-Wook (Department of Urology School of Medicine Kyungpook National University Daegu Korea.Department of Urology Kyungpook National University Chilgok Hospital Daegu Korea.) 윤보현 (경북대학교(의치대) 국제재생의학연구소) Jeon Minji (Joint Institute for Regenerative Medicine Kyungpook National University) Kim Hyun Tae (Department of Urology School of Medicine Kyungpook National University Daegu Korea.) Kwon Tae Gyun (Department of Urology Kyungpook National University Chilgok Hospital Kyungpook National University School of Medicine Daegu Korea.) Yoo Eun Sang (Department of Urology School of Medicine Kyungpook National University Daegu Korea.) Kim Bum Soo (Department of Urology Kyungpook National University Hospital Daegu Korea.Department of Urology School of Medicine Kyungpook National University Daegu Korea.)
저널정보
한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제20권 제2호
발행연도
2023.4
수록면
225 - 237 (13page)
DOI
10.1007/s13770-022-00505-w

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Background: Interstitial cystitis (IC) is a chronic and intractable disease that can severely deteriorate patients’ quality of life. Recently, stem cell therapy has been introduced as a promising alternative treatment for IC in animal models. We aimed to verify the efficacy and safety of the human perirenal adipose tissue-derived stromal vascular fraction (SVF) in an IC rat model. Methods: From eight-week-old female rats, an IC rat model was established by subcutaneous injection of 200 μg of uroplakin3A. The SVF was injected into the bladder submucosal layer of IC rats, and pain scale analysis, awakening cytometry, and histological and gene analyses of the bladder were performed. For the in vivo safety analysis, genomic DNA purification and histological analysis were also performed to check tumorigenicity and thrombus formation. Results: The mean pain scores in the SVF 20 μl group were significantly lower on days 7 and 14 than those in the control group, and bladder intercontraction intervals were significantly improved in the SVF groups in a dose-dependent manner. Regeneration of the bladder epithelium, basement membrane, and lamina propria was observed in the SVF group. In the SVF groups, however, bladder fibrosis and the expression of inflammatory markers were not significantly improved compared to those in the control group. Conclusion: This study demonstrated that a perirenal adipose tissue-derived SVF is a promising alternative for the management of IC in terms of improving bladder pain and overactivity.

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