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논문 기본 정보

자료유형
학술저널
저자정보
So Young Chun (BioMedical Research Institute Kyungpook National University Hospital Daegu Korea) Jeong Ok Lim (Kyungpook National University) Eun Hye Lee (School of Medicine Kyungpook National University) Man-Hoon Han (Department of Pathology Kyungpook National University Hospital Daegu Korea) Yun-Sok Ha (Department of Urology School of Medicine Kyungpook National University) Jun Nyung Lee (Kyungpook National University) Bum Soo Kim (Kyungpook National University Medical Center) Min Jeong Park (Daegu-Gyeongbuk Medical Innovation Foundation) 여명구 (대구경북첨단의료산업진흥재단) 정봉수 (대구경북첨단의료산업진흥재단) Tae Gyun Kwon (Kyungpook National University Hospital)
저널정보
한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제16권 제4호
발행연도
2019.1
수록면
385 - 393 (9page)

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BACKGROUND: Human adipose tissue is routinely discarded as medical waste. However, this tissue may have valuable clinical applications since methods have been devised to effectively isolate adipose-derived extracellular matrix (ECM), growth factors (GFs), and stem cells. In this review, we analyze the literature that devised these methods and then suggest an optimal method based on their characterization results. METHODS: Methods that we analyze in this article include: extraction of adipose tissue, decellularization, confirmation of decellularization, identification of residual active ingredients (ECM, GFs, and cells), removal of immunogens, and comparing structural/physiological/biochemical characteristics of active ingredients. RESULTS: Human adipose ECMs are composed of collagen type I–VII, laminin, fibronectin, elastin, and glycosaminoglycan (GAG). GFs immobilized in GAG include basic fibroblast growth factor (bFGF), transforming growth factor beta 1(TGF-b1), insulin like growth factor 1 (IGF-1), vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), BMP4 (bone morphogenetic protein 4), nerve growth factor (NGF), hepatocyte growth factor (HGF), and epithermal growth factor (EGF). Stem cells in the stromal-vascular fraction display mesenchymal markers, self-renewal gene expression, and multi-differentiation potential. CONCLUSION: Depending on the preparation method, the volume, biological activity, and physical properties of ECM, GFs, and adipose tissue-derived cells can vary. Thus, the optimal preparation method is dependent on the intended application of the adipose tissue-derived products.

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