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학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

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논문 기본 정보

자료유형
학술저널
저자정보
Salem Salah Abood (Department of Surgery UKM Medical Centre) Rashidbenam Zahra (Universiti Kebangsaan Malaysia Medical Centre) Jasman Mohd Hafidzul (Department of Surgery UKM Medical Centre) Ho Christopher Chee Kong (School of Medicine Taylor`s University) Sagap Ismail (Urology Unit Department of Surgery UKM Medical Centre) Singh Rajesh (Department of Orthopaedics and Traumatology UKM Medical Centre) Yusof Mohd Reusmaazran (Malaysian Nuclear Agency) Md. Zainuddin Zulkifli (Department of Surgery UKM Medical Centre) Haji Idrus Ruszymah Bt (Universiti Kebangsaan Malaysia Medical Centre) Ng Min Hwei (Universiti Kebangsaan Malaysia Medical Centre)
저널정보
한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제17권 제4호
발행연도
2020.1
수록면
553 - 563 (11page)

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Background: The urinary tract can be affected by both congenital abnormalities as well as acquired disorders, such as cancer, trauma, infection, inflammation, and iatrogenic injuries, all of which may lead to organ damage requiring eventual reconstruction. As a gold standard, gastrointestinal segment is used for urinary bladder reconstruction. However, one major problem is that while bladder tissue prevents reabsorption of specific solutes, gastrointestinal tissue actually absorbs them. Therefore, tissue engineering approach had been attempted to provide an alternative tissue graft for urinary bladder reconstruction. Methods: Human adipose-derived stem cells isolated from fat tissues were differentiated into smooth muscle cells and then seeded onto a triple-layered PLGA sheet to form a bladder construct. Adult athymic rats underwent subtotal urinary bladder resection and were divided into three treatment groups (n = 3): Group 1 (“sham”) underwent anastomosis of the remaining basal region, Group 2 underwent reconstruction with the cell-free scaffold, and Group 3 underwent reconstruction with the tissue-engineered bladder construct. Animals were monitored on a daily basis and euthanisation was performed whenever a decline in animal health was detected. Results: All animals in Groups 1, 2 and 3 survived for at least 7 days and were followed up to a maximum of 12 weeks post-operation. It was found that by Day 14, substantial ingrowth of smooth muscle and urothelial cells had occurred in Group 2 and 3. In the long-term follow up of group 3 (tissue-engineered bladder construct group), it was found that the urinary bladder wall was completely regenerated and bladder function was fully restored. Urodynamic and radiological evaluations of the reconstructed bladder showed a return to normal bladder volume and function.Histological analysis revealed the presence of three muscular layers and a urothelium similar to that of a normal bladder. Immunohistochemical staining using human-specific myocyte markers (myosin heavy chain and smoothelin) confirmed the incorporation of the seeded cells in the newly regenerated muscular layers. Conclusion: Implantation of PLGA construct seeded with smooth muscle cells derived from human adipose stem cells can lead to regeneration of the muscular layers and urothelial ingrowth, leading to formation of a completely functional urinary bladder.
#Bladder reconstruction #PLGA #Adipose-dervied stem cells #Tissue engineering #Smooth muscle cells

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참고문헌 (25)

참고문헌 신청
Yoo JJ / 1998 / Bladder augmentation using allogenic bladder submucosa seeded with cells / Urology 51:221~225 google schola Lewis SA / 2000 / Everything you wanted to know about the bladder epithelium but were afraid to ask / Am J Physiol Renal Physiol 278:F867~F874 google schola Soergel TM / 2004 / Transitional cell carcinoma of the bladder following augmentation cystoplasty for the neuropathic bladder / J Urol 172:1649~1651 google schola Lam Van Ba O / 2015 / Bladder tissue engineering: a literature review / Adv Drug Deliv Rev 82?83:31~37 google schola Atala A / 2011 / Tissue engineering of human bladder / Br Med Bull 97:81~104 google schola

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