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Anti-inflammatory Effects of Goihwa-san Water Extract via NF-κB Inhibition
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槐花散의 NF-κB 기전을 통한 항염증 효과 연구

논문 기본 정보

Type
Academic journal
Author
Hyun Hee Cho (가원한의원) Ji Young Choi (대구한의대 부속 대구한방병원 안이비인후피부과) Min Hwangbo (대구한의대 부속 대구한방병원 안이비인후피부과) Seon Young Jee (대구한의대 부속 대구한방병원 안이비인후피부과)
Journal
대한한방안이비인후피부과학회 한방안이비인후피부과학회지 한방안이비인후피부과학회지 제36권 제1호 KCI Accredited Journals
Published
2023.2
Pages
21 - 39 (19page)

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Anti-inflammatory Effects of Goihwa-san Water Extract via NF-κB Inhibition
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Objectives : The purpose of this study was to investigate the anti-inflammatory effect of Goihwa-san water extract(GHS) in vitro & in vivo. Methods : In vitro, we evaluated the anti-inflammatory effect of GHS by comparing the Raw 264.7 cells with 10, 30, 100, 300㎍/㎖ of GHS for 1 hour before Lipopolysaccharide(LPS) to the single LPS treated group. We examined the relative cell viability by MTT assay and the relative level of LPS, Loxoribine(LOX), Peptidoglycan(PGN), Flagellin(FLA)-induced NO production by using Griess reagent and measured relative iNOS protein level and COX-2 protein level by using western blot and Image analyzing system. We measured the production of TNF-α, IL-1β, and IL-6 by each ELISA kits and then measured the relative levels of IκBα, p-IκBα in whole-cell lysate fraction and NF-κB in nuclear fraction by using western blot and Image analyzing system. In vivo, we induced the paw edema by subcutaneous injection of 100㎕/rat CA and measured the swelling volume of paw by using a plethysmometer and then measured the relative iNOS protein level by using western blot. Results : As a result, in vitro, LPS, PGN-induced NO production was significantly inhibited by pretreatment with GHS. GHS reduced LPS, PGN-induced iNOS expression, PGN-induced COX-2 expression and LPS-induced production of cytokine(TNF-α, IL-1β, IL-6). Expression of IκBα was increased by pretreatment with GHS 100㎍/㎖. And the expression of p-IκBα and NF-κB were decreased by pretreatment with GHS 100㎍/㎖. In vivo, CA-induced inflammation rat model was used for the evaluation of the anti-inflammatory effect of GHS. 0.3 or 1.0g/㎏ of GHS significantly reduced the increases of paw swelling and iNOS expression in paw tissues. Conclusions : These results show that GHS can decrease inflammatory response via inhibition of the NF-κB pathway in vitro. And in vivo, the anti-inflammatory effect suggest the clinical basis of GHS for the treatment of inflammatory diseases.
#Anti-inflammatory effect #NF-κB #Goihwa-san

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