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논문 기본 정보

자료유형
학술저널
저자정보
Kim Nam-Yee (Division of Emerging Infectious Diseases Department of Diseases Research Incheon Metropolitan City Institute of Public Health and Environment Incheon Korea.) Shi Hye Jin (Division of Infectious Diseases Department of Internal Medicine Gil Medical Center Gachon University College of Medicine Incheon Korea.) Oh Sung-Suck (Division of Emerging Infectious Diseases Department of Diseases Research Incheon Metropolitan City Institute of Public Health and Environment Incheon Korea.) Gong Young-Woo (Division of Emerging Infectious Diseases Department of Diseases Research Incheon Metropolitan City Institute of Public Health and Environment Incheon Korea.) Kwon Mun-Ju (Division of Emerging Infectious Diseases Department of Diseases Research Incheon Metropolitan City Institute of Public Health and Environment Incheon Korea.) Eom Joong Sik (Division of Infectious Diseases Department of Internal Medicine Gil Medical Center Gachon University College of Medicine Incheon Korea.) Park Yoonseon (Division of Infectious Diseases Department of Internal Medicine Gil Medical Center Gachon University College of Medicine Incheon Korea.)
저널정보
대한감염학회 Infection and Chemotherapy Infection and Chemotherapy 제55권 제2호
발행연도
2023.6
수록면
214 - 225 (12page)
DOI
10.3947/ic.2022.0152

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Background We aimed to identify the genes of 35 pathogens, including viruses, bacteria, and protozoa that cause waterborne infectious diseases, and to assess the feasibility of a wastewater-based surveillance system. Materials and Methods Wastewater was aseptically sampled once a month from 2 sites. A total of 1 L of wastewater from each site underwent 0.2 µm filtration to generate the sample A. Subsequently, 200 ul of the filtered water was ultra-filtered and concentrated to generate the sample B, which was mixed with sample A in a 1:1 ratio. We performed a Filmarray® Gastrointestinal (GI) panel (BioFire Diagnnostics’, Salt Lake City, UT, USA) test to simultaneously detect 13 enterobacteria, 5 enterovirus, and 4 protozoa. RNA was extracted to assess 18 types of viruses. Results Severe acute respiratory syndrome coronavirus 2 adenovirus, bocavirus, and rhinovirus was detected at both site. Norovirus GI/GII was continuously detected at both sites. Moreover, adenovirus, group A rotaviruses, and hepatitis A virus were frequently detected; however, hepatitis E virus was absent at either site. Campylobacter, enteroaggregative Escherichia coli, enterotoxigenic E. coli, Shiga toxin-producing E. coli, and Clostridioides difficile toxin A/B were detected at both sites. Giardia lamblia was also detected in both sites. Conclusion We analyze multiple infectious disease pathogens under sample surveillance with incidence. Further in-depth studies on wastewater-based surveillance will be feasible and important.

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