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Future Perspectives on New Approaches in Pathogen Detection
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Type
Academic journal
Author
Peng Li (Singapore Polytechnic) Bow Ho (National University of Singapore) Jeak Ling Ding (National University of Singapore)
Journal
The Korean Society For Biomedical Laboratory Sciences Biomedical Science Letters Vol.21 No.4 KCI Accredited Journals
Published
2015.12
Pages
165 - 171 (7page)

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Future Perspectives on New Approaches in Pathogen Detection
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Abstract· Keywords

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Microbial pathogens are responsible for most of the rapidly-spreading deadly infectious diseases against humans. Thus, there is an urgent need for efficient and rapid detection methods for infectious microorganisms. The detection methods should not only be targeted and specific, but they have to be encompassing of potential changes of the pathogen as it evolves and mutates quickly during an epidemic or pandemic. The existing diagnostics such as the antibody-based ELISA immunoassay and PCR methods are too selective and narrowly focused; they are insufficient to capture newly evolved mutant strains of the pathogen. Here, we introduce a fresh perspective on some new technologies, including aptamers and next generation sequencing for pathogen detection. These technologies are not in their infancy; they are reasonably mature and ready, and they hold great promise for unparalleled applications in pathogen detection.
#Pathogen detection #Pathogen mutation #Antibiotic resistance #Aptamers #Next generation sequencing

Contents

INTRODUCTION
Can aptamers provide more superior pathogen diagnostics than antibodies?
Next Generation Sequencing, a robust tool in pathogen gene detection/identification
CONCLUSION
REFERENCES

References (36)

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[학술지(정기간행물)] - Abbaspour A / 2015 / Aptamerconjugated silver nanoparticles for electrochemical dualaptamer-based sandwich detection of Staphylococcus aureus / Biosens Bioelectron 68 : 149 ~ 155 google schola [학술지(정기간행물)] - Acquah C / 2015 / A review on immobilised aptamers for high throughput biomolecular detection and screening / Anal Chim Acta 888 : 10 ~ 18 google schola [학술지(정기간행물)] - Akira S / 2001 / Toll-like receptors: critical proteins linking innate and acquired immunity / Nat Immunol 2 : 675 ~ 680 google schola [학술지(정기간행물)] - Bruno JG / 2015 / Predicting the uncertain future of aptamer-based diagnostics and therapeutics / Molecules 20 : 6866 ~ 6887 google schola [학술지(정기간행물)] - Cho S / 2005 / Bis-aptazyme sensors for hepatitis C virus replicase and helicase without blank signal / Nucleic Acids Res 33 : e177 ~ google schola

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