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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 220 - 225 (6page)
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초록· 키워드
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Background: Diarrhea has been the second leading cause of death among children under the age of five, and the rapid and accurate pathogen diagnosis in patients with diarrhea is crucial for reducing morbidity and mortality. A newly developed one-step multiplex realtime PCR assay, the Allplex GI-Virus Assay, was evaluated for its ability to detect six diarrhea- causing viruses (rotavirus, norovirus genogroup I (GI) and genogroup II (GII), enteric adenovirus, astrovirus, and sapovirus) in stool samples. Methods: The performance of the Allplex assay was compared with those of another multiplex PCR assay (Seeplex Diarrhea-V Ace Detection) and genotyping by sequencing, using 446 stool samples from patients with acute gastroenteritis. Results: The overall agreement rates between the results of the Allplex and Seeplex assays were 98.7% for rotavirus, 99.1% for norovirus GI, 93.3% for norovirus GII, 98.0% for adenovirus, and 99.6% for astrovirus. The overall agreement rates between the Allplex assay and genotyping were 99.1% for rotavirus, 99.1% for norovirus GI, 98.7% for norovirus GII, 89.7% for adenovirus, 98.2% for astrovirus, and 99.8% for sapovirus. In addition, eight rotavirus genotypes, three norovirus GI genotypes, four norovirus GII genotypes, eight adenovirus genotypes, two astrovirus genotypes, and two sapovirus genotypes were detected. Conclusions: The Allplex assay showed high agreement with Seeplex and genotyping results, and was able to additionally detect sapoviruses. The Allplex assay could be useful in identifying viral gastrointestinal infections in patients with acute gastroenteritis symptoms.
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참고문헌 (17)
참고문헌 신청
Liu L / 2012 / Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000 / Lancet 379:2151~2161
Liu J / 2011 / Multiplex reverse transcription PCR Luminex assay for detection and quantitation of viral agents of gastroenteritis / J Clin Virol 50:308~313
Bennett S / 2016 / The development of a multiplex real-time RT-PCR for the detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples / J Virol Methods 242:30~34
Feng W / 2015 / The application and epidemiological research of xTAG GPP multiplex PCR in the diagnosis of infectious diarrhea / Zhonghua Yi Xue Za Zhi 95:435~439
Higgins RR / 2011 / Evaluation and verification of the Seeplex Diarrhea-V ACE assay for simultaneous detection of adenovirus, rotavirus, and norovirus genogroups I and II in clinical stool specimens / J Clin Microbiol 49:3154~3162
Liu J / 2011 / Multiplex reverse transcription PCR Luminex assay for detection and quantitation of viral agents of gastroenteritis / J Clin Virol 50:308~313
Bennett S / 2016 / The development of a multiplex real-time RT-PCR for the detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples / J Virol Methods 242:30~34
Feng W / 2015 / The application and epidemiological research of xTAG GPP multiplex PCR in the diagnosis of infectious diarrhea / Zhonghua Yi Xue Za Zhi 95:435~439
Higgins RR / 2011 / Evaluation and verification of the Seeplex Diarrhea-V ACE assay for simultaneous detection of adenovirus, rotavirus, and norovirus genogroups I and II in clinical stool specimens / J Clin Microbiol 49:3154~3162
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