Heterologous Expression and Characterization of a Novel Exo-Polygalacturonase from Aspergillus fumigatus Af293 and Its Application in Juice Extraction

Yang Chengwei; Zhang Ting; Zhu Jing; Wei Yunyi; Zhu Furong; Cheng Zhong

doi:10.4014/jmb.2211.11003

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자료유형: 학술저널

저자정보: Yang Chengwei (College of Food Science and Quality Engineering Nanning University Nanning Guangxi 530200 P.R. ChinaCollege of Life Science and Technology Guangxi University Nanning Guangxi 530004 P.R. China) Zhang Ting (College of Food Science and Quality Engineering Nanning University Nanning Guangxi 530200 P.R. China) Zhu Jing (College of Food Science and Quality Engineering Nanning University Nanning Guangxi 530200 P.R. China) Wei Yunyi (College of Food Science and Quality Engineering Nanning University Nanning Guangxi 530200 P.R. China) Zhu Furong (College of Food Science and Quality Engineering Nanning University Nanning Guangxi 530200 P.R. China) Cheng Zhong (College of Food Science and Quality Engineering Nanning University Nanning Guangxi 530200 P.R. China)

저널정보: 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제33권 제4호

발행연도: 2023.4

수록면: 533 - 542 (10page)

DOI: 10.4014/jmb.2211.11003

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Exo-polygalacturonase (exo-PG) hydrolyzes pectin acids and liberates mono-galacturonate, which plays an important role in juice extraction, and has rarely been reported. Exo-PG (AfumExoPG28A) from Aspergillus fumigatus belongs to the glycoside hydrolase 28 family. In this study, its gene was cloned and the protein was expressed and secreted in Pichia pastoris with a maximal activity of 4.44 U/ml. The optimal temperature and pH of AfumExoPG28A were 55°C and 4.0, respectively. The enzyme exhibited activity over almost the entire acidic pH range (>20.0% activity at pH 2.5-6.5) and remained stable at pH 2.5–10.0 for 24 h. The Km and Vmax values of AfumExoPG28A were calculated by the substrate of polygalacturonic acid as 25.4 mg/ml and 23.6 U/mg, respectively. Addition of AfumExoPG28A (0.8 U/mg) increased the light transmittance and juice yield of plantain pulp by 11.7% and 9%, respectively. Combining AfumExoPG28A (0.8 U/mg) with an endo-PG (0.8 U/mg) from our laboratory, the enzymes increased the light transmittance and juice yield of plantain pulp by 45.7% and 10%, respectively. Thus, the enzyme’s potential value in juice production was revealed by the remarkable acidic properties and catalytic activity in fruit pulp.

#Exo-polygalacturonase #Aspergillus fumigatus #acid stability #juice extraction

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이 논문의 저자 정보

Yang Chengwei

소속기관 College of Food Science and Quality Engineering Nanning University Nanning Guangxi 530200 P.R. ChinaCollege of Life Science and Technology Guangxi University Nanning Guangxi 530004 P.R. China

주요연구분야 자연과학 > 생물학

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Zhang Ting