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논문 기본 정보

자료유형
학술저널
저자정보
Lu Bo (National Engineering Research Center for Non-Food Biorefinery Guangxi Key Laboratory of Biorefinery) Xian Liang (National Engineering Research Center for Non-Food Biorefinery Guangxi Key Laboratory of Biorefinery) Zhu Jing (Nanning University 8 Longting Road Nanning Guangxi 530200 P.R. China) Wei Yunyi (Nanning University 8 Longting Road Nanning Guangxi 530200 P.R. China) Yang Chengwei (Nanning University 8 Longting Road Nanning Guangxi 530200 P.R. China) Cheng Zhong (Nanning University 8 Longting Road Nanning Guangxi 530200 P.R. China)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제4호
발행연도
2022.4
수록면
464 - 472 (9page)
DOI
10.4014/jmb.2112.12023

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An endo-polygalacturonase (endo-PGase) exhibiting excellent performance during acidic fruit juice production would be highly attractive to the fruit juice industry. However, candidate endo-PGases for this purpose have rarely been reported. In this study, we expressed a gene from Penicillium oxalicum in Pichia pastoris. The recombinant enzyme PoxaEnPG28C had an optimal enzyme activity at pH 4.5 and 45°C and was stable at pH 3.0?6.5 and < 45°C. The enzyme had a specific activity of 4,377.65 ± 55.37 U/mg towards polygalacturonic acid, and the Km and Vmax values of PoxaEnPG28C were calculated as 1.64 g/l and 6127.45 U/mg, respectively. PoxaEnPG28C increased the light transmittance of orange, lemon, strawberry and hawthorn juice by 13.9 ± 0.3%, 29.4 ± 3.8%, 95.7 ± 10.2% and 79.8 ± 1.7%, respectively; it reduced the viscosity of the same juices by 25.7 ± 1.6%, 52.0 ± 4.5%, 48.2 ± 0.7% and 80.5 ± 2.3%, respectively, and it increased the yield of the juices by 24.5 ± 0.7%, 12.7 ± 2.2%, 48.5 ± 4.2% and 104.5 ± 6.4%, respectively. Thus, PoxaEnPG28C could be considered an excellent candidate enzyme for acidic fruit juice production. Remarkably, fruit juice production using hawthorn as an material was reported for the first time.

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