지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.3
- 수록면
- 218 - 227 (10page)
이용수
초록· 키워드
오류제보하기
Background: Myocardial fibrosis (MF) is an advanced pathological manifestation of many cardiovascular diseases, which can induce heart failure and malignant arrhythmias. However, the current treatment of MF lacks specific drugs. Ginsenoside Re has anti-MF effect in rat, but its mechanism is still not clear. Therefore, we investigated the anti-MF effect of ginsenoside Re by constructing mouse acute myocardial infarction (AMI) model and AngII induced cardiac fibroblasts (CFs) model.
Methods: The anti-MF effect of miR-489 was investigated by transfection of miR-489 mimic and inhibitor in CFs. Effect of ginsenoside Re on MF and its related mechanisms were investigated by ultrasonographic, ELISA, histopathologic staining, transwell test, immunofluorescence, Western blot and qPCR in the mouse model of AMI and the AngII-induced CFs model. Results: MiR-489 decreased the expression of a-SMA, collagenI, collagen III and myd88, and inhibited the phosphorylation of NF-kB p65 in normal CFs and CFs treated with AngII. Ginsenoside Re could improve cardiac function, inhibit collagen deposition and CFs migration, promote the transcription of miR-489, and reduce the expression of myd88 and the phosphorylation of NF-kB p65.
Conclusion: MiR-489 can effectively inhibit the pathological process of MF, and the mechanism is at least partly related to the regulation of myd88/NF-kB pathway. Ginsenoside Re can ameliorate AMI and AngII induced MF, and the mechanism is at least partially related to the regulation of miR-489/myd88/NF-kB signaling pathway. Therefore, miR-489 may be a potential target of anti-MF and ginsenoside Re may be an effective drug for the treatment of MF.
Methods: The anti-MF effect of miR-489 was investigated by transfection of miR-489 mimic and inhibitor in CFs. Effect of ginsenoside Re on MF and its related mechanisms were investigated by ultrasonographic, ELISA, histopathologic staining, transwell test, immunofluorescence, Western blot and qPCR in the mouse model of AMI and the AngII-induced CFs model. Results: MiR-489 decreased the expression of a-SMA, collagenI, collagen III and myd88, and inhibited the phosphorylation of NF-kB p65 in normal CFs and CFs treated with AngII. Ginsenoside Re could improve cardiac function, inhibit collagen deposition and CFs migration, promote the transcription of miR-489, and reduce the expression of myd88 and the phosphorylation of NF-kB p65.
Conclusion: MiR-489 can effectively inhibit the pathological process of MF, and the mechanism is at least partly related to the regulation of myd88/NF-kB pathway. Ginsenoside Re can ameliorate AMI and AngII induced MF, and the mechanism is at least partially related to the regulation of miR-489/myd88/NF-kB signaling pathway. Therefore, miR-489 may be a potential target of anti-MF and ginsenoside Re may be an effective drug for the treatment of MF.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
5. Conclusions
References
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
miR-196b-5p and miR-375 Expression in Gastric Cancer
대한임상종양학회 학술대회지
2016 .02
-
Down Regulation of miR-34a and miR-143 May Indirectly Inhibit p53 in Oral Squamous Cell Carcinoma: a Pilot Study
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Circulating Plasma Micro RNAs in Patients with Major Depressive Disorder Treated with Antidepressants: A Pilot Study
PSYCHIATRY INVESTIGATION
2016 .01
-
Effects of lipid nanoparticles on physicochemical properties, cellular uptake, and lymphatic uptake of 6-methoxflavone
Journal of Pharmaceutical Investigation
2022 .03
-
Triptolide improves myocardial fibrosis in rats through inhibition of nuclear factor kappa B and NLR family pyrin domain containing 3 inflammasome pathway
The Korean Journal of Physiology & Pharmacology
2021 .10
-
Regulation of Colonic Mucosal MicroRNA Expression via Multiple Targets in Visceral Hypersensitivity Rats by Tongxieyaofang
Yonsei Medical Journal
2018 .01
-
Upregulation of miR-27b Facilitates Apoptosis of TNF-α-Stimulated Fibroblast-Like Synoviocytes
Yonsei Medical Journal
2019 .01
-
Investigation of Dysregulation of Several MicroRNAs in Peripheral Blood of Schizophrenia Patients
Clinical Psychopharmacology and Neuroscience
2016 .01
-
Exercise induced Right Ventricular Fibrosis is Associated with Myocardial Damage and Inflammation
Korean Circulation Journal
2018 .01
-
A novel panel of serum miR-21/miR-155/miR-365 as a potential diagnostic biomarker for breast cancer
Annals of Surgical Treatment and Research
2017 .02
-
MiR-675 Promotes the Growth of Hepatocellular Carcinoma Cells Through Cdc25A Pathway
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
MiR-484 Protects Rat Myocardial Cells from Ischemia-Reperfusion Injury by Inhibiting Caspase-3 and Caspase-9 during Apoptosis
Korean Circulation Journal
2020 .01
-
MiR-92a-1-5p and miR-328-3p Are Up-Regulated in Skin of Female Pattern Hair Loss Patients
Annals of Dermatology
2019 .01
-
MicroRNA Expression in Plasma of Esophageal Squamous Cell Carcinoma Patients
Journal of Korean Medical Science
2022 .06
-
Exosomes derived from miR-214-3p overexpressing mesenchymal stem cells promote myocardial repair
생체재료학회지
2023 .03
-
Expression of miR-221 and miR-18a in patients with hepatocellular carcinoma and its clinical significance
KOREAN JOURNAL OF CLINICAL ONCOLOGY
2022 .06
-
Combined Detection of Serum MiR-221-3p and MiR-122-5p Expression in Diagnosis and Prognosis of Gastric Cancer
Journal of Gastric Cancer
2019 .01
-
miR-195/miR-497 Regulate CD274 Expression of Immune Regulatory Ligands in Triple-Negative Breast Cancer
Journal of Breast Cancer
2018 .01
-
Circulating miR-221 and miR-222 as Potential Biomarkers for Screening of Breast Cancer
대한의생명과학회지
2019 .06
-
Expression of specific microRNAs in tissue and plasma in colorectal cancer
Journal of Pathology and Translational Medicine
2023 .05
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2023-524-001465844