Simultaneous detection of aflatoxin B1, deoxynivalenol, and fumonisin B1 in blood with liquid chromatography-tandem mass spectrometry

Kwang-Nam Kim; Soohee Kim; Hyobi Kim; Seo-Il Yang; Heesu Kim; Jae-Young Song; Sung-Won Park

doi:http://dx.doi.org/10.13041/jpvm.2015.39.3.108

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자료유형: 학술저널

저자정보: Kwang-Nam Kim (Animal and Plant Quarantine Agency) Soohee Kim (Animal and Plant Quarantine Agency) Hyobi Kim (Animal and Plant Quarantine Agency) Seo-Il Yang (Animal and Plant Quarantine Agency) Heesu Kim (Animal and Plant Quarantine Agency) Jae-Young Song (Animal and Plant Quarantine Agency) Sung-Won Park (Animal and Plant Quarantine Agency)

저널정보: 한국예방수의학회 예방수의학회지 예방수의학회지 제39권 제3호

발행연도: 2015.9

수록면: 108 - 113 (6page)

DOI: http://dx.doi.org/10.13041/jpvm.2015.39.3.108

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Mycotoxins, such as aflatoxin B1 (AFB1), deoxynivalenol (DON), fumonisin B1 (FMB1), ochratoxin A, T2 toxin, and zearalenone, are found in numerous vegetables. Mycotoxin accumulation in food and feed poses serious health risks to humans and animals because of carcinogenic, mutagenic, teratogenic, and toxic properties. In addition, mycotoxins cause large economic losses in commercial crop production, food and feed processing, and animal husbandry worldwide. In this study, an analytical method for the simultaneous analysis of the levels of AFB1, DON, and FMB1 in cow blood with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated. AOZTM and Myco6in1TM multitoxin immunoaffinity columns and an OasisTM reversed-phase solid-phase extraction Hydrophilic-Lipophilic-Balanced columns were used to purify and concentrate the blood samples. Extracts that contained AFB1, DON, and FMB1 had average recovery of 64.0%, 98.0%, and 89.9%, respectively. In conclusion, we used LC-MS/MS to detect several important toxicological mycotoxins in cow blood. The multimycotoxin method, which detected and quantified the levels of AFB1, DON, and FMB1 can be used in animal pilot studies to monitor simultaneous exposure to major mycotoxins.

#Mycotoxins #cow blood #multitoxin immunoaffinity column #LC-MS/MS

참고문헌 (14)

참고문헌 신청

Bennett JW / 2003 / Mycotoxins / Clin Microbiol Rev 16:497~516

Birzele B / 2000 / Deoxynivalenil and ochratoxin A in German wheat and changes of level in relation to storage parameters / Food Addit Contam 17:1027~1035

Calvano F / 2005 / Mycotoxins in the human food chain / Nottingham University Press :187~224

Dall’Asta C / 2008 / A LC/MS/MS method for the simultaneous quantification of free and masked fumonisins in maizebased products / World Mycotoxin J 1:237~246

Decastelli L / 2007 / Aflatoxins occurrence in milk and feed in Northern Italy during 2004-2005 / Food Control 18:1263~1266

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