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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 59 - 62 (4page)
- DOI
- 10.34184/kssb.2021.9.4.59
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초록· 키워드
오류제보하기
Burosumab is a first-in-class therapy approved to treat X-linked hypophosphatemia and tumor-induced osteomalacia, a condition associated with excessive fibroblast growth factor 23 (FGF23) production. This antibody drug directly targets the excessive FGF23 in patients and inhibits its regulation of phosphate homeostasis and vitamin D metabolism. Here, the Fab fragment of burosumab was expressed, purified, and crystallized. The crystals belonged to the space group P21, with unit cell parameters a = 49.51, b = 66.22, c = 62.05 A, and β = 105.99°. An asymmetric unit of the crystal contains one Fab fragment with a Matthews coefficient of 1.93 A3 Da?1. The structure was determined at a resolution of 1.45 A, with Rwork/Rfree = 0.169/0.196. The complementarity determining regions of burosumab construct a negatively charged patch, a putative hot spot for FGF23 binding. This high-resolution structure could be used for predicting the binding mode between burosumab and FGF23.
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등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
Adams, P. D. / 2010 / PHENIX : a comprehensive Python-based system for macromolecular structure solution / Acta Crystallogr D Biol Crystallogr 66:213~221
ADHR Consortium / 2000 / Autosomal dominant hypophosphataemic rickets is associated with mutations in FGF23 / Nat Genet 26:345~348
Chen, G. / 2018 / α-Klotho is a non-enzymatic molecular scaffold for FGF23 hormone signalling / Nature 553:461~466
Emsley, P. / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
Goetz, R. / 2007 / Molecular insights into the klotho-dependent, endocrine mode of action of fibroblast growth factor 19 subfamily members / Mol Cell Biol 27:3417~3428
ADHR Consortium / 2000 / Autosomal dominant hypophosphataemic rickets is associated with mutations in FGF23 / Nat Genet 26:345~348
Chen, G. / 2018 / α-Klotho is a non-enzymatic molecular scaffold for FGF23 hormone signalling / Nature 553:461~466
Emsley, P. / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
Goetz, R. / 2007 / Molecular insights into the klotho-dependent, endocrine mode of action of fibroblast growth factor 19 subfamily members / Mol Cell Biol 27:3417~3428
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