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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.2
- 수록면
- 110 - 118 (9page)
- DOI
- 10.14348/molcells.2018.2210
이용수
초록· 키워드
오류제보하기
The objective of this study was to induce the production of isthmic organizer (IsO)-like cells capable of secreting fibroblast growth factor (FGF) 8 and WNT1 from human embryonic stem cells (ESCs). The precise modulation of canonical Wnt signaling was achieved in the presence of the small molecule CHIR99021 (0.6 ?M) during the neural induction of human ESCs, resulting in the differentiation of these cells into IsO-like cells having a midbrain-hindbrain border (MHB) fate in a manner that recapitulated their developmental course in vivo. Resultant cells showed upregulated expression levels of FGF8 and WNT1. The addition of exogenous FGF8 further increased WNT1 expression by 2.6 fold. Gene ontology following microarray analysis confirmed that IsO-like cells enriched the expression of MHB-related genes by 40 fold compared to control cells. Lysates and conditioned media of IsO-like cells contained functional FGF8 and WNT1 proteins that could induce MHB-related genes in differentiating ESCs. The method for generating functional IsO-like cells described in this study could be used to study human central nervous system development and congenital malformations of the midbrain and hindbrain.
목차
등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Barkovich, A.J. / 2009 / A developmental and genetic classification for midbrain-hindbrain malformations / Brain 132:3199~3230
Basson, M.A. / 2013 / Congenital hypoplasia of the cerebellum: developmental causes and behavioral consequences / Front. Neuroanat. 7:29
Benjamini, Y. / 2001 / The control of the false discovery rate in multiple testing under dependency / Ann. Stat. 29:1165~1188
Blaess, S. / 2015 / Genetic control of midbrain dopaminergic neuron development / Wiley Interdisciplinary Reviews -Developmental Biology 4:113~134
Broccoli, V. / 1999 / The caudal limit of Otx2 expression positions the isthmic organizer / Nature 401:164~168
Basson, M.A. / 2013 / Congenital hypoplasia of the cerebellum: developmental causes and behavioral consequences / Front. Neuroanat. 7:29
Benjamini, Y. / 2001 / The control of the false discovery rate in multiple testing under dependency / Ann. Stat. 29:1165~1188
Blaess, S. / 2015 / Genetic control of midbrain dopaminergic neuron development / Wiley Interdisciplinary Reviews -Developmental Biology 4:113~134
Broccoli, V. / 1999 / The caudal limit of Otx2 expression positions the isthmic organizer / Nature 401:164~168
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