지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
오류제보하기
Dual specificity phosphatases (DUSPs) include MAP kinase phosphatases and atypical dual specificity phosphatases,and mediate cell growth and differentiation. They are considered as drug targets against cancers, diabetes, immunediseases, and neuronal diseases. Two different DUSPs, DUSP13a and DUSP13b are coded in one gene (DUSP13) whosealternative splicing results in two sequence-related proteins with different target specificities. The crystal structure ofDUSP13b showed a canonical DUSP fold. However, the structure of DUSP13a has not been determined yet. To understandstructural mechanism of distinctive target specificities of sequence-related DUSPs, we prepared diffraction-quality crystalsof DUSP13a. Cysteine residues in DUSP13a gene were mutated to serine or alanine to prevent cysteine oxidation. Fromthe stabilized protein, we were able to grow good crystals that diffracted to 1.7 A resolution. The preliminary diffractionanalysis revealed that the crystal is in the space group P21 with unit cell parameters of a = 40.04 A, b = 89.34 A, c = 45.90 A,α = 90.00°, β = 89.87° and γ = 90.00°.
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참고문헌 (16)
참고문헌 신청
Alonso, A. / 2016 / The extended human PTPome: a growing tyrosine phosphatase family / FEBS J 283:1404~1429
Bermudez, O. / 2010 / The dual-specificity MAP kinase phosphatases: critical roles in development and cancer / Am J Physiol Cell Physiol 299:C189~C202
Chen, H.H. / 2004 / Characterization of two distinct dual specificity phosphatases encoded in alternative open reading frames of a single gene located on human chromosome 10q22.2 / J Biol Chem 279:41404~41413
Jeong, D.G. / 2014 / The family-wide structure and function of human dual-specificity protein phosphatases / Acta Crystallogr D Biol Crystallogr 70:421~435
Katagiri, C. / 2011 / DUSP13B/TMDP inhibits stress-activated MAPKs and suppresses AP-1-dependent gene expression / Mol Cell Biochem 352:155~162
Bermudez, O. / 2010 / The dual-specificity MAP kinase phosphatases: critical roles in development and cancer / Am J Physiol Cell Physiol 299:C189~C202
Chen, H.H. / 2004 / Characterization of two distinct dual specificity phosphatases encoded in alternative open reading frames of a single gene located on human chromosome 10q22.2 / J Biol Chem 279:41404~41413
Jeong, D.G. / 2014 / The family-wide structure and function of human dual-specificity protein phosphatases / Acta Crystallogr D Biol Crystallogr 70:421~435
Katagiri, C. / 2011 / DUSP13B/TMDP inhibits stress-activated MAPKs and suppresses AP-1-dependent gene expression / Mol Cell Biochem 352:155~162
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