지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충북대학교 동물의학연구소 Journal of Biomedical and Translational Research Journal of Biomedical and Translational Research 제19권 제1호
- 발행연도
- 2018.3
- 수록면
- 16 - 20 (5page)
이용수
초록· 키워드
오류제보하기
Mycoplasma (M.) felis and M. canis is related with pneumonia or conjunctivitis in domestic cats and several diseases in a variety of other animals, including lower respiratory tract disease or pleuritis. Polymerase chain reaction (PCR) assays has been reported as an easy and useful method. It could be conducted even on nasal swab samples as a non-invasive rapid testing tools for large numbers of Mycoplasma species. However, PCR assays have to conduct multiple assays because of a lot of Mycoplasma species. Therefore, it need to perform several tests and reveal time consuming procedures. In this study, we developed a sensitive and specific multiplex polymerase chain reaction (PCR) assay that detects simultaneously two species like as M. felis and M. canis. The simultaneous detection of M. felis and M. canis primers were used to differentiate two mycoplasma species. The target DNA fragments were specifically amplified M. felis and M. canis PCR with 16S ribosomal DNA primers. Single and mixed Mycoplasma species DNA templates were submitted to validate the specificity of the multiplex PCR. The corresponding specific DNA products were amplified for each pathogen. The detection limit of the developed multiplex PCR is 102 pg with M. felis and M. canis DNA. Furthermore, the developed multiplex PCR detected successfully M. felis in feline nasal specimens. The multiplex PCR assay provides a novel tool for simultaneous detection and differentiation of M. felis and M. canis in cats.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Hay RJ / 1989 / Mycoplasma infection of cultured cells / Nature 339:487~488
김옥진 / 2004 / Application of Consensus Polymerase Chain Reaction for Monitoring of Helicobactrer Species / Laboratory Animal Research 20(4):316~320
Hopert AC / 1993 / Specificity and sensitivity of polymerase chain reaction (PCR) in comparison with other methods for the detection of mycoplasma contamination in cell lines / Journal of Immunological Methods 164:91~100
Tang J / 2000 / A polymerase chain reaction based method for detecting Mycoplasma/Acholeplasma contaminants in cell culture / Journal of Microbiological Methods 39:121~126
Harasawa R / 1993 / Detection and tentative identification of dominant Mycoplasma species in cell cultures by restriction analysis of the 16S-23S rRNA intergenic spacer regions / Research in Microbiology 144:489~493
김옥진 / 2004 / Application of Consensus Polymerase Chain Reaction for Monitoring of Helicobactrer Species / Laboratory Animal Research 20(4):316~320
Hopert AC / 1993 / Specificity and sensitivity of polymerase chain reaction (PCR) in comparison with other methods for the detection of mycoplasma contamination in cell lines / Journal of Immunological Methods 164:91~100
Tang J / 2000 / A polymerase chain reaction based method for detecting Mycoplasma/Acholeplasma contaminants in cell culture / Journal of Microbiological Methods 39:121~126
Harasawa R / 1993 / Detection and tentative identification of dominant Mycoplasma species in cell cultures by restriction analysis of the 16S-23S rRNA intergenic spacer regions / Research in Microbiology 144:489~493
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Molecular diagnosis of Mycoplasma felis from the kenneled cats
한국실험동물학회 학술발표대회 논문집
2015 .08
-
Detection of Mycoplasma felis from the kenneled cats with pneumonia
한국가축위생학회지
2015 .01
-
Prevalence of Bordetella bronchiseptica, Mycoplasma felis, and Chlamydia felis using a newly developed triplex real-time polymerase chain reaction assay in Korean cat population
한국가축위생학회지
2022 .12
-
Detection and molecular characterization of Hepatozoon canis, Babesia vogeli, Ehrlichia canis, and Anaplasma platys in dogs from Metro Manila, Philippines
Korean Journal of Veterinary Research(대한수의학회지)
2017 .08
-
Comparison of the non-invasive diagnostic methods, stool antigen test and PCR assay, for Helicobacter felis detection in dogs
한국가축위생학회지
2015 .01
-
Investigation of chlamydophilosis from naturally infected cats
Journal of Veterinary Science
2021 .11
-
Development of a rapid isolation of Mycoplasma species in laboratory animals
한국실험동물학회 학술발표대회 논문집
2018 .07
-
Development of a rapid isolation and culture method of wild type Mycoplasma spp. in pigs
Journal of Biomedical and Translational Research
2018 .06
-
Anti-inflammatory and Anti-tumorigenic Effects of Açai Berry in Helicobacter felis-infected mice
대한암예방학회지
2016 .01
-
Non-invasive diagnostic methods for Helicobacter felis detection in animals
한국실험동물학회 학술발표대회 논문집
2015 .08
-
Identification of Pasteurella canis in a Soft Tissue Infection Caused by a Dog Bite: The First Report in Korea
Annals of Laboratory Medicine
2016 .01
-
Multiplex Real-Time PCR Method for Simultaneous Identification and Toxigenic Type Characterization of Clostridium difficile From Stool Samples
Annals of Laboratory Medicine
2015 .01
-
Virulence-associated Genome Sequences of Pasteurella canis and Unique Toxin Gene Prevalence of P. canis and Pasteurella multocida Isolated from Humans and Companion Animals
Annals of Laboratory Medicine
2023 .05
-
First Case of Canine Infection with Hepatozoon canis (Apicomplexa: Haemogregarinidae) in the Republic of Korea
Parasites, Hosts and Diseases
2017 .10
-
A triplex real-time PCR assay for simultaneous and differential detection of Bordetella bronchiseptica, Mycoplasma cynos, and Mycoplasma canis in respiratory diseased dogs
한국가축위생학회지
2023 .03
-
A newly-developed polymerase chain reaction to detect Mycoplasma pulmonis in laboratory animals
한국실험동물학회 학술발표대회 논문집
2015 .08
-
Evaluation of Multiplex Polymerase Chain Reaction Assay for the Simultaneous Detection of Sexually Transmitted Infections Using Swab Specimen
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2020 .03
-
Effect of N-Methyl-N-Nitrosourea on Helicobacter-induced Gastric Carcinogenesis in C57BL/6 Mice
대한암예방학회지
2016 .01
-
NLRP3 inflammasome mediates IL-1β production in response to Helicobacter felis in immune cells of dogs
한국실험동물학회 학술발표대회 논문집
2017 .08
-
Mycoplasma Infection as a Cause of Persistent Fever after Intravenous Immunoglobulin Treatment of Patients with Kawasaki Disease: Frequency and Clinical Impact
Infection and Chemotherapy
2017 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0