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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 37 - 42 (6page)
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초록· 키워드
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The aim of the present study was to compare the non-invasive methods for the diagnosis of H. feliswith HpSA kit-based detection method and H. felis-specific PCR assay with dog’s stool samples withoutsacrifice. Male Beagle dogs (n=6) were infected with H. felis ATCC 49179 (1.0×109 CFU/dog) by intragastricinoculation two times at 3-day intervals, and the stool specimens of dogs were collected 1, 3,5, 7, 14, 21 days after infection to submit to HpSA test and H. felis-specific PCR. As the results, thesensitivity of the HpSA and the PCR analysis was 50.0%, 83.3% respectively. Although HpSA test isless sensitive, it could be used for rapid, cheap and easy screening assay for H. felis infection in dogand cats. We suggest that the H. pylori stool antigen kit, HpSA, is useful and effective for monitoringH. felis infection. If HpSA test would be made with H. felis antibodies in the future, its sensitivity couldbe increased. Also, PCR assay could be successfully used to detect the H. felis in stools. Applying theH. pylori stool antigen kit and PCR assay may be the recommended non-invasive strategy to identifyH. felis in dog and cats.
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등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Demirturk L / 2003 / Does N-acetyl cystein affect the sensitivity and specificity of Helicobacter pylori stool antigen test? / Helicobacter 8 : 120 ~ 123
Eaton KA / 1991 / Essential role of urease in pathogenesis of gastritis induced by Helicobacter pylori in gnotobiotic piglets / Infect Immun 59 : 2470 ~ 2475
Fox JG / 1995 / Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice / J Clin Microbiol 33 : 445 ~ 454
Hodzic E / 2001 / Evaluation of diagnostic methods for Helicobacter bilis infection in laboratory mice / Comp Med 51 : 406 ~ 412
Hoshina S / 1990 / Direct detection and amplification of Helicobacter pylori ribosomal 16S gene segments from gastric endoscopic biopsies / Diagn Microbiol Infect Dis 13 : 47
Eaton KA / 1991 / Essential role of urease in pathogenesis of gastritis induced by Helicobacter pylori in gnotobiotic piglets / Infect Immun 59 : 2470 ~ 2475
Fox JG / 1995 / Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice / J Clin Microbiol 33 : 445 ~ 454
Hodzic E / 2001 / Evaluation of diagnostic methods for Helicobacter bilis infection in laboratory mice / Comp Med 51 : 406 ~ 412
Hoshina S / 1990 / Direct detection and amplification of Helicobacter pylori ribosomal 16S gene segments from gastric endoscopic biopsies / Diagn Microbiol Infect Dis 13 : 47
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