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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충북대학교 동물의학연구소 Journal of Biomedical and Translational Research Journal of Biomedical and Translational Research 제19권 제2호
- 발행연도
- 2018.6
- 수록면
- 38 - 42 (5page)
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초록· 키워드
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Mycoplasma spp. are extracellular bacteria that colonize on the respiratory epithelium of humans and animals. It is a causative agent of pneumonia commonly complicated by opportunistic infectious bacteria. Mycoplasma spp. infection cause relatively mild disease in the absence of environmental stressors, but when complicated by secondary bacterial invaders the resultant disease can cause obvious clinical disease and severe production losses in intensively reared pigs Mycoplasma spp. are highly fastidious bacteria, difficult to culture and slow growing. Many species of Mycoplasma spp. are important pathogens causing respiratory infection in animals and known to induce huge economic losses. The aims of the present study were to develop a rapid isolation and culture method of wild type Mycoplasma spp. in pigs. We used Mycoplasma spp. genus specific direct PCR without DNA extraction procedure using Phireⓡ Animal Tissue Direct PCR Kit from the lung tissues with pneumonia lesions. Therefore, we could save the time for tissue processing and increase the accuracy of Mycoplasma spp. inclusion prediction in lung tissues. Thereafter, we used the optimized media to isolate and culture Mycoplasma spp. As the results, Mycoplasma spp. could be isolated and cultured quickly and efficiently. These results could provide an efficient strategy and method for the rapid and accurate isolation and culture of wild type Mycoplasma spp. in pigs.
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등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Kobisch M / 1996 / Swine mycoplasmoses / Rev Sci Tech 15:1569~1605
홍선화 / 2015 / Diagnosis of Mycoplasma hyorhinis infection in pigs by PCR amplification of 16S-23S rRNA internal transcribed spacer region / Journal of Biomedical and Translational Research 16(3):104~108
Villarreal I / 2011 / Effect of vaccination of pigs against experimental infection with high and low virulence Mycoplasma hyopneumoniae strains / Vaccine 29:1731~1735
Friis NF / 1994 / Mycoplasma hyorhinis in the etiology of serositis among piglets / Acta Vet Scand 35:93~98
Razin S / 1998 / Molecular biology and pathogenicity of mycoplasmas / Microbiol Mol Biol Rev 62:1094~1156
홍선화 / 2015 / Diagnosis of Mycoplasma hyorhinis infection in pigs by PCR amplification of 16S-23S rRNA internal transcribed spacer region / Journal of Biomedical and Translational Research 16(3):104~108
Villarreal I / 2011 / Effect of vaccination of pigs against experimental infection with high and low virulence Mycoplasma hyopneumoniae strains / Vaccine 29:1731~1735
Friis NF / 1994 / Mycoplasma hyorhinis in the etiology of serositis among piglets / Acta Vet Scand 35:93~98
Razin S / 1998 / Molecular biology and pathogenicity of mycoplasmas / Microbiol Mol Biol Rev 62:1094~1156
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