지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 851 - 862 (12page)
- DOI
- 10.1007/s13770-021-00350-3
이용수
초록· 키워드
오류제보하기
Background: Stem cell therapies can be a new therapeutic strategy that may rebalance anabolic and anti-resorptive effects in osteoporosis patients. Tonsil-derived mesenchymal stem cells (TMSCs) can be an alternative therapeutic source for chronic degenerative diseases including osteoporosis. MSCs acquire immune regulatory function under the inflammatory cytokines. Since interleukin (IL) 1β is known to be one of inflammatory cytokines involved in osteoporosis progression, treatment of IL1β with TMSCs may enhance immunomodulatory function and therapeutic effects of TMSCs in osteoporosis.
Methods: For IL1β priming, TMSCs were cultured in the presence of the medium containing IL1β for 1 day. Characteristics of IL1β priming TMSCs such as multipotent differentiation properties, anti-inflammatory potential, and suppression of osteoclast differentiation were assessed in vitro. For in vivo efficacy study, IL1β priming TMSCs were intravenously infused twice with ovariectomized (OVX) osteoporosis mouse model, and blood serum and bone parameters from micro computed tomography images were analyzed.
Results: IL1β priming TMSCs had an enhanced osteogenic differentiation and secreted factors that regulate both osteoclastogenesis and osteoblastogenesis. IL1β priming TMSCs also suppressed proliferation of peripheral blood mononuclear cells (PBMCs) and decreased expression of Receptor activator of nuclear factor kappa-Β ligand (RANKL) in PHA-stimulated PBMCs. Furthermore, osteoclast specific genes such as Nuclear factor of activated T cells c1 (NFATc1) were effectively down regulated when co-cultured with IL1β priming TMSCs in RANKL induced osteoclasts. In OVX mice, IL1β priming TMSCs induced low level of serum RANKL/osteoprotegerin (OPG) ratio on the first day of the last administration. Four weeks after the last administration, bone mineral density and serum Gla-osteocalcin were increased in IL1β priming TMSC-treated OVX mice. Furthermore, bone formation and bone resorption markers that had been decreased in OVX mice with low calcium diet were recovered by infusion of IL1β priming TMSCs.
Conclusion: IL1β priming can endow constant therapeutic efficacy with TMSCs, which may contribute to improve bone density and maintain bone homeostasis in postmenopausal osteoporosis. Therefore, IL1β priming TMSCs can be a new therapeutic option for treating postmenopausal osteoporosis.
목차
등록된 정보가 없습니다.
참고문헌 (44)
참고문헌 신청
Sozen T / 2017 / An overview and management of osteoporosis / Eur J Rheumatol 4:46~56
Marx RE / 2003 / Pamidronate(Aredia)and zoledronate(Zometa)induced avascular necrosis of the jaws : a growing epidemic / J Oral Maxillofac Surg 61:1115~1117
Rossouw JE / 2002 / Risks and benefits of estrogen plus progestin in healthy postmenopausal women : principal results From the Women’s Health Initiative randomized controlled trial / JAMA 288:321~333
Vahle JL / 2002 / Skeletal changes in rats given daily subcutaneous injections of recombinant human parathyroid hormone(1–34)for 2 years and relevance to human safety / Toxicol Pathol 30:312~321
Zullo F / 2003 / Effectiveness of presacral neurectomy in women with severe dysmenorrhea caused by endometriosis who were treated with laparoscopic conservative surgery : a 1-year prospective randomized double-blind controlled trial / Am J Obstet Gynecol 189:5~10
Marx RE / 2003 / Pamidronate(Aredia)and zoledronate(Zometa)induced avascular necrosis of the jaws : a growing epidemic / J Oral Maxillofac Surg 61:1115~1117
Rossouw JE / 2002 / Risks and benefits of estrogen plus progestin in healthy postmenopausal women : principal results From the Women’s Health Initiative randomized controlled trial / JAMA 288:321~333
Vahle JL / 2002 / Skeletal changes in rats given daily subcutaneous injections of recombinant human parathyroid hormone(1–34)for 2 years and relevance to human safety / Toxicol Pathol 30:312~321
Zullo F / 2003 / Effectiveness of presacral neurectomy in women with severe dysmenorrhea caused by endometriosis who were treated with laparoscopic conservative surgery : a 1-year prospective randomized double-blind controlled trial / Am J Obstet Gynecol 189:5~10
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Matrix Metalloproteinase 1 as a Marker of Tonsil-Derived Mesenchymal Stem Cells to Assess Bone Marrow Cell Migration
조직공학과 재생의학
2023 .04
-
Basic Fibroblast Growth Factor Induces Cholinergic Differentiation of Tonsil-Derived Mesenchymal Stem Cells
조직공학과 재생의학
2022 .10
-
A Novel Method to Differentiate Tonsil-Derived Mesenchymal Stem Cells In Vitro into Estrogen-Secreting Cells
조직공학과 재생의학
2021 .01
-
Expression of Tenocyte Lineage-Related Factors from Tonsil-Derived Mesenchymal Stem Cells
조직공학과 재생의학
2016 .01
-
Intracellular Remodeling and Accumulation of Aberrant Lysosomes in Differentiation of Tonsil-Derived Mesenchymal Stem Cells into Parathyroid-Like Cells
조직공학과 재생의학
2017 .01
-
Regenerative Potential of Tonsil-derived Mesenchymal Stem Cells Using Hydrogel Systems
한국생물공학회 학술대회
2022 .04
-
Wound Healing Promoting Activity of Tonsil-Derived Stem Cells on 5-Fluorouracil-Induced Oral Mucositis Model
조직공학과 재생의학
2020 .01
-
Optimization of Microenvironments Inducing Differentiation of Tonsil-Derived Mesenchymal Stem Cells into Endothelial Cell-Like Cells
조직공학과 재생의학
2019 .01
-
Pluronic pyridinyl dicarboxylate thermogel for 3D culture of TMSC
한국고분자학회 학술대회 연구논문 초록집
2018 .04
-
Galactosylated polypeptide thermogel as an injectable scaffold of the TMSCs for hepatogenic differentiation
한국고분자학회 학술대회 연구논문 초록집
2017 .10
-
IL-30† (IL-27A): a familiar stranger in immunity, inflammation, and cancer
Experimental and Molecular Medicine
2021 .05
-
IL-33 promotes IL-10 production in macrophages: a role for IL-33 in macrophage foam cell formation
Experimental and Molecular Medicine
2017 .01
-
Exosomes from IL-1β-Primed Mesenchymal Stem Cells Inhibited IL-1β- and TNF-α-Mediated Inflammatory Responses in Osteoarthritic SW982 Cells
조직공학과 재생의학
2021 .01
-
A neutrophil elastase-generated mature form of IL-33 is a potent regulator of endothelial cell activation and proliferative retinopathy
Experimental and Molecular Medicine
2024 .08
-
Effect of TNF-α and IL-6 on Compact Bone-Derived Cells
조직공학과 재생의학
2021 .01
-
Locus of (IL-9) control: IL9 epigenetic regulation in cellular function and human disease
Experimental and Molecular Medicine
2024 .06
-
Bolstering the secretion and bioactivities of umbilical cord MSC-derived extracellular vesicles with 3D culture and priming in chemically defined media
Nano Convergence
2022 .12
-
Interleukin-32: Frenemy in cancer?
BMB Reports
2019 .01
-
Harnessing IL-2 for immunotherapy against cancer and chronic infection: A historical perspective and emerging trends
Experimental and Molecular Medicine
2024 .09
-
Interleukin-20 targets podocytes and is upregulated in experimental murine diabetic nephropathy
Experimental and Molecular Medicine
2017 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0