지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 411 - 420 (10page)
이용수
초록· 키워드
오류제보하기
Differentiation of mesenchymal stem cells (MSC) into a variety of cell lineages such as adipocytes, osteocytes, and chondrocytes is often accompanied up-regulation of autophagy. In our study, we demonstrated that the expression of autophagy-associated proteins (p-Beclin 1, LC3A, LC3B, p-AMPK, p-mTOR and ATG3, ATG7, and ATG12-5) over a period of time was hardly distinguishable from control tonsil-derived MSC (TMSC). Despite the unnoticeable difference in autophagy activation between differentiated TMSC (dTMSC) and the control (cTMSC), we reported significant changes in intracellular compositions in differentiated TMSC into functional parathyroid-like cells secreting parathyroid hormone (PTH). By using transmission electron microscopy (TEM), we observed accumulation of multivesicular bodies (MVB) comprising small, degraded compartments densely accumulated as dark granular or amorphous clumps, multilamellar bodies and lipid droplets in dTMSC. However, no such structures were found in cTMSC. These results suggest that differentiation of TMSC into parathyroid-like cells producing PTH hormone is hardly dependent on autophagy activation in the beginning of our conditions. Furthermore, our results of intracellular remodeling and accumulated endo-lysosomal storage bodies in the later stages of TMSC differentiation present a possible role of the structures in PTH secretion.
목차
등록된 정보가 없습니다.
참고문헌 (32)
참고문헌 신청
Mizushima N / 2011 / The role of Atg proteins in autophagosome formation / Annu Rev Cell Dev Biol 27:107~132
He C / 2009 / Regulation mechanisms and signaling pathways of autophagy / Annu Rev Genet 43:67~93
Huang R / 2015 / Identifying an essential role of nuclear LC3 for autophagy / Autophagy 11:852~853
Tanida I / 2004 / LC3 conjugation system in mammalian autophagy / Int J Biochem Cell Biol 36:2503~2518
Phadwal K / 2013 / Tightrope act: autophagy in stem cell renewal, differentiation, proliferation, and aging / Cell Mol Life Sci 70:89~103
He C / 2009 / Regulation mechanisms and signaling pathways of autophagy / Annu Rev Genet 43:67~93
Huang R / 2015 / Identifying an essential role of nuclear LC3 for autophagy / Autophagy 11:852~853
Tanida I / 2004 / LC3 conjugation system in mammalian autophagy / Int J Biochem Cell Biol 36:2503~2518
Phadwal K / 2013 / Tightrope act: autophagy in stem cell renewal, differentiation, proliferation, and aging / Cell Mol Life Sci 70:89~103
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Basic Fibroblast Growth Factor Induces Cholinergic Differentiation of Tonsil-Derived Mesenchymal Stem Cells
조직공학과 재생의학
2022 .10
-
Regenerative Potential of Tonsil-derived Mesenchymal Stem Cells Using Hydrogel Systems
한국생물공학회 학술대회
2022 .04
-
Matrix Metalloproteinase 1 as a Marker of Tonsil-Derived Mesenchymal Stem Cells to Assess Bone Marrow Cell Migration
조직공학과 재생의학
2023 .04
-
A Novel Method to Differentiate Tonsil-Derived Mesenchymal Stem Cells In Vitro into Estrogen-Secreting Cells
조직공학과 재생의학
2021 .01
-
Therapeutic Effect of IL1β Priming Tonsil Derived-Mesenchymal Stem Cells in Osteoporosis
조직공학과 재생의학
2021 .10
-
Expression of Tenocyte Lineage-Related Factors from Tonsil-Derived Mesenchymal Stem Cells
조직공학과 재생의학
2016 .01
-
Wound Healing Promoting Activity of Tonsil-Derived Stem Cells on 5-Fluorouracil-Induced Oral Mucositis Model
조직공학과 재생의학
2020 .01
-
Screening of Single-stranded DNA Aptamers for Specific Parathyroid Hormone Aptamers for Specific Parathyroid Hormone
한국생물공학회 학술대회
2021 .04
-
Optimization of Microenvironments Inducing Differentiation of Tonsil-Derived Mesenchymal Stem Cells into Endothelial Cell-Like Cells
조직공학과 재생의학
2019 .01
-
MiR-449a attenuates autophagy of T-cell lymphoma cells by downregulating ATG4B expression
BMB Reports
2020 .01
-
Selection and Docking-simulation of Single-stranded DNA Aptamers for Specific Parathyroid Hormone
한국생물공학회 학술대회
2021 .10
-
Effect of Low Temperature on Schwann-Like Cell Differentiation of Bone Marrow Mesenchymal Stem Cells
조직공학과 재생의학
2015 .01
-
Galactosylated polypeptide thermogel as an injectable scaffold of the TMSCs for hepatogenic differentiation
한국고분자학회 학술대회 연구논문 초록집
2017 .10
-
Structure biology of selective autophagy receptors
BMB Reports
2016 .01
-
The interplay of microRNAs and transcription factors in autophagy regulation in nonalcoholic fatty liver disease
Experimental and Molecular Medicine
2021 .04
-
Role of the mammalian ATG8/LC3 family in autophagy: differential and compensatory roles in the spatiotemporal regulation of autophagy
BMB Reports
2016 .01
-
Quantitative and qualitative analysis of autophagy flux using imaging
BMB Reports
2020 .01
-
Effect of Size-controlled Graphene Oxide Sheet on Differentiation of Mesenchymal Stem Cells
한국생물공학회 학술대회
2017 .10
-
Polarized light emitting diode enhanced neuronal differentiation in tonsil derived mesenchymal stem cell
한국고분자학회 학술대회 연구논문 초록집
2016 .10
-
Pluronic pyridinyl dicarboxylate thermogel for 3D culture of TMSC
한국고분자학회 학술대회 연구논문 초록집
2018 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0