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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 162 - 170 (9page)
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Human palatine tonsil-derived mesenchymal stem cells (TMSCs) are known to be a new source of progenitor cells. Using waste tissue after tonsillectomy as a cell provider can be the biggest benefit of TMSCs, compared with other stem cells. The purpose of this study was to investigate tenogenic differentiation of TMSCs and to access the differential effects of transforming growth factor beta 3 (TGF-β3) on the tenogenesis of TMSCs. Human tonsil was obtained after tonsillectomy. Using a cytometric analysis, we were able to find that the TMSCs had typical mesenchymal stem cell markers: positive for CD73, CD90, and CD105, and negative for CD14, CD34, and CD45. Using TGF-β3, the expressions of tenocyte-specific genes and proteins, such as collagen type 1 (COL1), tenomodulin (TNMD), and scleraxis (SCX), were measured by a quantitative polymerase chain reaction (PCR), immunofluorescence staining, immunohistochemistry and Western blot analyses. Quantitative PCR assay showed that TGF-β3 significantly increased the expressions of tenocyte lineage marker genes, including COL1, TNMD, and SCX, at a 3-day treatment, compared with control. However, these increases were not found at long-term exposures (7 or 10 days), except that TNMD expression was maintained at 50 ng/mL at a 7-day exposure to TGF-β3. Like genes, the protein expression levels of COL1, TNMD, and SCX were also induced in TGF-β3-treated TMSCs in a 3-day treatment, which were maintained for 10 days, as evidenced by immunofluorescence staining, immunohistochemistry and Western blot analyses. This study demonstrated that TMSCs in tenogenic stimulation with TGF-β3 have a high tenogenic differentiation potential.
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참고문헌 (48)
참고문헌 신청
Oliva F / 2014 / Epidemiology of the rotator cuff tears: a new incidence related to thyroid disease / Muscles Ligaments Tendons J 4:309~314
Ganestam A / 2015 / Increasing incidence of acute Achilles tendon rupture and a noticeable decline in surgical treatment from 1994 to 2013. A nationwide registry study of 33,160 patients / Knee Surg Sports Traumatol Arthrosc
Iannotti JP / 2013 / Time to failure after rotator cuff repair: a prospective imaging study / J Bone Joint Surg Am 95:965~971
Clanton T / 2014 / Mann’s surgery of the foot and ankle / Saunders :1621~1642
Gerber C / 2000 / The results of repair of massive tears of the rotator cuff / J Bone Joint Surg Am 82:505~515
Ganestam A / 2015 / Increasing incidence of acute Achilles tendon rupture and a noticeable decline in surgical treatment from 1994 to 2013. A nationwide registry study of 33,160 patients / Knee Surg Sports Traumatol Arthrosc
Iannotti JP / 2013 / Time to failure after rotator cuff repair: a prospective imaging study / J Bone Joint Surg Am 95:965~971
Clanton T / 2014 / Mann’s surgery of the foot and ankle / Saunders :1621~1642
Gerber C / 2000 / The results of repair of massive tears of the rotator cuff / J Bone Joint Surg Am 82:505~515
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