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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 227 - 232 (6page)
- DOI
- 10.17135/jdhs.2021.21.4.227
이용수
초록· 키워드
오류제보하기
Background: Cancer-associated fibroblasts (CAFs) are abundant in tumor microenvironments and interact with cancer cells to promote tumor proliferation in oral squamous cell carcinoma (OSCC). Cathepsin D (CTSD) is a soluble lysosomal aspartic endopeptidase involved in tumor proliferation and angiogenesis. In this preliminary study, we observed CTSD expression in OSCC and CAFs, postulating that CTSD might act as a bridge between OSCC and CAFs.
Methods: Human epidermal keratinocytes (HEKs), OSCC, and immortalized human normal oral fibroblasts (hTERT-hNOFs) were used in this study. Additionally, we used hTERT-hNOFs transfected with an empty vector, WT (wild-type)-YAP (Yes-associated protein), and YAPS127A (YAP serine 127 to alanine). YAP127A hTERT-hNOFs activated fibroblasts similar to CAFs. To identify CTSD expression between OSCC and CAFs, conditioned medium (CM) was collected from each cell. Protein expression of CTSD was identified by western blotting.
Results: To identify the expression of CTSD in fibroblasts stimulated by OSCC, we treated fibroblasts with CM from HEK and OSCC. Results indicated that hTERT-hNOFs with OSCC CM showed a weakly increased expression of CTSD compared to stimulation by HEK CM. This indicates that CAFs, YAPS127 hTRET-hNOFs, overexpress CTSD protein. HEK cells showed no CTSD expression, regardless of treatment with fibroblast CM, whereas OSCC highly expressed CTSD proteins compared with the CTSD expression in HEK cells. We also found that CTSD expression was unaffected by changes in transforming growth factor-β levels.
Conclusion: This study proposes that CTSD might have potential as an interacting executor between OSCC and CAFs. Further studies are needed to investigate the role of CTSD in tumor and stromal cells.
목차
등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
Roghayyeh Baghban / 2020 / Tumor microenvironment complexity and therapeutic implications at a glance / Cell Communication and Signaling 18(1):59
Kenneth C. Valkenburg / 2018 / Targeting the tumour stroma to improve cancer therapy / Nature Reviews Clinical Oncology 15(6):366~381
Raghu Kalluri / 2006 / Fibroblasts in cancer / Nature Reviews Cancer 6(5):392~401
Zehuan Liao / 2019 / Cancer-associated fibroblasts in tumor microenvironment – Accomplices in tumor malignancy / Cellular Immunology 343:103729
A. Calon / 2014 / TGF-beta in CAF-mediated tumor growth and metastasis / Seminars in Cancer Biology 25:15~22
Kenneth C. Valkenburg / 2018 / Targeting the tumour stroma to improve cancer therapy / Nature Reviews Clinical Oncology 15(6):366~381
Raghu Kalluri / 2006 / Fibroblasts in cancer / Nature Reviews Cancer 6(5):392~401
Zehuan Liao / 2019 / Cancer-associated fibroblasts in tumor microenvironment – Accomplices in tumor malignancy / Cellular Immunology 343:103729
A. Calon / 2014 / TGF-beta in CAF-mediated tumor growth and metastasis / Seminars in Cancer Biology 25:15~22
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