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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 140 - 145 (6page)
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초록· 키워드
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This study aimed to develop Lautropia mirabilis -specific quantitative real-time polymerase chain reaction (qPCR) primers based on the sequence of DNA-directed RNA polymerase subunit beta gene. The PrimerSelect program was used in designing of the qPCR primers, RTLam-F4 and RTLam-R3. The specificity of the qPCR primers were performed by conventional PCR with 37 strains of 37 oral bacterial species, including L. mirabilis . The sensitivity of the primers was determined by qPCR with the serial dilution of purified genomic DNA of L. mirabilis KCOM 3484, ranged from 4 ng to 4 fg. The data showed that the qPCR primers could detect only L. mirabilis strains and as little as 40 fg of genome DNA of L. mirabilis KCOM 3484. These results indicate that this qPCR primer pair (RTLam-F4/ RTLam-R3) may be useful for species-specific detection of L. mirabilis in epidemiological studies of oral bacterial infectious diseases such as periodontal disease.
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참고문헌 (13)
참고문헌 신청
Gerner-Smidt P / 1994 / Lautropia mirabilis gen. nov., sp. nov., a gram-negative motile coccus with unusual morphology isolated from the human mouth / Microbiology (Reading) 140(Pt 7):1787~1797
Lim YK / 2019 / isolated from human dental plaque of a gingivitis lesion / Curr Microbiol 76:1369~1373
Colombo AP / 2009 / Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray / J Periodontol 80:1421~1432
Colombo AP / 2012 / Impact of periodontal therapy on the subgingival microbiota of severe periodontitis : comparison between good responders and individuals with refractory periodontitis using the human oral microbe identification microarray / J Periodontol 83:1279~1287
Shaddox LM / 2012 / Microbiological characterization in children with aggressive periodontitis / J Dent Res 91:927~933
Lim YK / 2019 / isolated from human dental plaque of a gingivitis lesion / Curr Microbiol 76:1369~1373
Colombo AP / 2009 / Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray / J Periodontol 80:1421~1432
Colombo AP / 2012 / Impact of periodontal therapy on the subgingival microbiota of severe periodontitis : comparison between good responders and individuals with refractory periodontitis using the human oral microbe identification microarray / J Periodontol 83:1279~1287
Shaddox LM / 2012 / Microbiological characterization in children with aggressive periodontitis / J Dent Res 91:927~933
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