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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 143 - 147 (5page)
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초록· 키워드
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In a previous study, Peptoniphilus mikwangii was isolated from the human oral cavity as a new species. The purpose of this study was to develop P. mikwangii-specific PCR primers.
The PCR primers were designed, based on the nucleotide sequence of 16S ribosomal RNA (16S rDNA). The specificity of the primers was tested using genomic DNAs of 3 strains of P. mikwangii and 27 strains (27 species) of non-P. mikwangii bacteria. The sensitivity of primers sensitivity was determined using PCR, with serial dilutions of the purified genomic DNAs (4 ng to 4 fg) of P. mikwangii KCOM 1628T. The data showed that P. mikwangii-specific qPCR primers (B134-F11/B134- R1 & B134-F5/B134-R5) could detect only P. mikwangii strains, and 400 fg or 40 fg of P. mikwangii genome DNA.
These results suggest that PCR primers are useful in detecting P. mikwangii from the oral cavity.
목차
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참고문헌 (13)
참고문헌 신청
Ashimoto A / 1996 / Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions / Oral Micr
E. Cho / 2013 / Draft Genome Sequence of the Novel Peptoniphilus sp. Strain ChDC B134, Isolated from a Human Periapical Abscess Lesion / Genome Announcements 1 (5) : e00822-
Eugene Cho / 2015 / Peptoniphilus mikwangii sp. nov., Isolated from a Clinical Specimen of Human Origin / Current Microbiology 70 (2) : 260 ~ 266
Ezaki T / 201 / Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus / Int J Syst Evol M
Hwan Seon Shin / 2010 / Development of strain-specific PCR primers for the identification of Fusobacterium nucleatum subsp. fusiforme ATCC 51190T and subsp. vincentii ATCC 4
E. Cho / 2013 / Draft Genome Sequence of the Novel Peptoniphilus sp. Strain ChDC B134, Isolated from a Human Periapical Abscess Lesion / Genome Announcements 1 (5) : e00822-
Eugene Cho / 2015 / Peptoniphilus mikwangii sp. nov., Isolated from a Clinical Specimen of Human Origin / Current Microbiology 70 (2) : 260 ~ 266
Ezaki T / 201 / Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus / Int J Syst Evol M
Hwan Seon Shin / 2010 / Development of strain-specific PCR primers for the identification of Fusobacterium nucleatum subsp. fusiforme ATCC 51190T and subsp. vincentii ATCC 4
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