Ardipusilloside-I stimulates gastrointestinal motility and phosphorylation of smooth muscle myosin by myosin light chain kinase

Zhili Xu; Hanye Liang; Mingbo Zhang; Xiaojun Tao; De-qiang Dou; Liping Hu; Tingguo Kang

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자료유형: 학술저널

저자정보: Zhili Xu (Liaoning University of Traditional Chinese Medicine) Hanye Liang (Liaoning University of Traditional Chinese Medicine) Mingbo Zhang (Liaoning University of Traditional Chinese Medicine) Xiaojun Tao (Liaoning University of Traditional Chinese Medicine) De-qiang Dou (Liaoning University of Traditional Chinese Medicine) Liping Hu (Liaoning University of Traditional Chinese Medicine) Tingguo Kang (Liaoning University of Traditional Chinese Medicine)

저널정보: 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제21권 제6호

발행연도: 2017.11

수록면: 609 - 616 (8page)

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Ardipusilloside-I is a natural triterpenoid saponin, which was isolated from Ardisia pusilla A. DC. The aim of the study was to evaluate the stimulation of ardipusilloside-I on gastrointestinal motility in vitro and in vivo. The experiment of smooth muscle contraction directly monitored the contractions of the isolated jejunal segment (IJS) in different contractile states, and the effects of ardipusilloside-I on myosin were measured in the presence of Ca2+-calmodulin using the activities of 20 kDa myosin light chain (MLC20) phosphorylation and myosin Mg2+-ATPase. The effects of ardipusilloside-I on gastro emptying and intestinal transit in constipation-predominant rats were observed, and the MLCK expression in jejuna of constipated rats was determined by western blot. The results showed that, ardipusilloside-I increased the contractility of IJS in a dose-dependent manner and reversed the low contractile state (LCS) of IJS induced by low Ca2+, adrenaline, and atropine respectively. There were synergistic effects on contractivity of IJS between ardipusilloside-I and ACh, high Ca2+, and histamine, respectively. Ardipusilloside-I could stimulate the phosphorylation of MLC20 and Mg2+-ATPase activities of Ca2+- dependent phosphorylated myosin. Ardipusilloside-I also stimulated the gastric emptying and intestinal transit in normal and constipated rats in vivo, respectively, and increased the MLCK expression in the jejuna of constipation-predominant rats. Briefly, the findings demonstrated that ardipusilloside-I could effectively excite gastrointestinal motility in vitro and in vivo.

#Ardipusilloside-I Gastrointestinal motility Myosin light chain kinase Myosin Mg2+-ATPase

참고문헌 (26)

참고문헌 신청

Zhang QH / 1993 / Studies on the sapogenins and prosapogenins in Ardisia pusilla A. DC / Zhongguo Zhong Yao Za Zhi 18:545

Cao WY / 2015 / Ardipusilloside-I metabolites from human intestinal bacteria and their antitumor activity / Molecules 20:20569~20581

Xu XF / 2013 / Ardipusilloside I induces apoptosis by regulating Bcl-2family proteins in human mucoepidermoid carcinoma Mc3 cells / BMC Complement Altern Med 13:322

Tao XJ / 2005 / Antitumor and immunological regulation effects of ardipusilloside-I / Chinese Pharmacological Bulletin 21:1070~1073

Vale RD / 2000 / The way things move : looking under the hood of molecular motor proteins / Science 288:88~95

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