지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 1,003 - 1,009 (7page)
- DOI
- 10.1007/s13258-021-01104-0
이용수
초록· 키워드
오류제보하기
Background The heart is one of the target organs vulnerable to sepsis. About 50% of sepsis patients will sufer from myocardial injury and cardiac dysfunction, which will aggravate the sepsis and afect its prognosis.
Objectives Here, we attempt to investigate the function of long non coding RNA PVT1 in LPS-induced cardiac fbroblasts in vitro, and explore its potential mechanism.
Methods The expression of PVT1 in LPS-induced cardiac fbroblasts was detected by qRT-PCR. CCK-8 assay, cell migration, qRT-PCR and western blotting analysis were applied to evaluating the efect of PVT1 knockdown on LPS-induced cardiac fbroblasts. The bioinformatics analysis and the rescue experiment were devoted to the underlying mechanism.
Results PVT1 expression was up-regulated in LPS-induced cardiac fbroblasts. And knockdown of PVT1 inhibited cell viability and migration, alleviated infammation cytokines production of LPS-treated cardiac fbroblasts. The bioinformatics analysis predicted PVT1 negatively regulates miR-24 and KLF6 is a direct target of miR-24.
Conclusions In a word, we observed PVT1 expression level was up-regulated in LPS- treated cardiac fbroblasts. PVT1 knockdown could alleviate LPS-induced biological behavior of cardiac fbroblasts through sponging miR-24 in vitro.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Antonucci E / 2014 / Myocardial depression in sepsis : from pathogenesis to clinical manifestations and treatment / J Crit Care 29:500~511
Bechmann LP / 2013 / Post-transcriptional activation of PPAR alpha by KLF6 in hepatic steatosis / J Hepatol 58:1000~1006
Bernard K / 2000 / Transcriptional expression analysis of T-cell activation by multiplex messenger assay(MMA) / Methods Mol Biol 134:337~353
Brown RD / 2005 / The cardiac fbroblast : therapeutic target in myocardial remodeling and failure / Annu Rev Pharmacol Toxicol 45:657~687
Chen JX / 2019 / Silence of long noncoding RNA NEAT1 exerts suppressive efects on immunity during sepsis by promoting microRNA-125-dependent MCEMP1 downregulation / IUBMB Life 71:956~968
Bechmann LP / 2013 / Post-transcriptional activation of PPAR alpha by KLF6 in hepatic steatosis / J Hepatol 58:1000~1006
Bernard K / 2000 / Transcriptional expression analysis of T-cell activation by multiplex messenger assay(MMA) / Methods Mol Biol 134:337~353
Brown RD / 2005 / The cardiac fbroblast : therapeutic target in myocardial remodeling and failure / Annu Rev Pharmacol Toxicol 45:657~687
Chen JX / 2019 / Silence of long noncoding RNA NEAT1 exerts suppressive efects on immunity during sepsis by promoting microRNA-125-dependent MCEMP1 downregulation / IUBMB Life 71:956~968
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