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논문 기본 정보

자료유형
학술저널
저자정보
Shangdi Zhang (Lanzhou University Second Hospital) Jinming Wang (Chinese Academy of Agricultural Sciences) Xiaoyun Li (Chinese Academy of Agricultural Sciences) Yanbo Wang (Lanzhou University Second Hospital) Yueli Nian (Lanzhou University Second Hospital) Chongge You (Lanzhou University Second Hospital) Dekui Zhang (Lanzhou University Second Hospital) Guiquan Guan (Chinese Academy of Agricultural Sciences)
저널정보
대한기생충학열대의학회 Parasites, Hosts and Diseases Parasites, Hosts and Diseases Vol.61 No.1
발행연도
2023.2
수록면
42 - 53 (12page)

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The genus Babesia includes parasites that can induce human and animal babesiosis, which are common in tropical and subtropical regions of the world. The gut microbiota has not been examined in hamsters infected by Babesia duncani. Red blood cells infected with B. duncani were injected into hamsters through intraperitoneal route. To evaluate the changes in gut microbiota, DNAs were extracted from small intestinal contents, acquired from hamsters during disease development. Then, the V4 region of the 16S rRNA gene of bacteria was sequenced using the Illumina sequencing platform. Gut microbiota alternation and composition were assessed according to the sequencing data, which were clustered with >97.0% sequence similarity to create amplicon sequence variants (ASVs). Bacteroidetes and Firmicutes were made up of the major components of the gut microbiota in all samples. The abundance of Bacteroidetes elevated after B. duncani infection than the B. duncani-free group, while Firmicutes and Desulfobacterota declined. Alpha diversity analysis demonstrated that the shown ASVs were substantially decreased in the highest parasitemia group than B. duncani-free and lower parasitemia groups. Potential biomarkers were discovered by Linear discriminant analysis Effect Size (LEfSe) analysis, which demonstrated that several bacterial families (including Muribaculaceae, Desulfovibrionaceae, Oscillospiraceae, Helicobacteraceae, Clostridia UGG014, Desulfovibrionaceae, and Lachnospiraceae) were potential biomarkers in B. duncani-infected hamsters. This research demonstrated that B. duncani infectious can modify the gut microbiota of hamsters.

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Abstract
Introduction
Materials and Methods
Results
Discussion
References

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