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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.3
- 수록면
- 20 - 24 (5page)
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초록· 키워드
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Cyanidin-3-glucoside (C3G) and cyanidin-3-rutinoside (C3R) were isolated by high-performance countercurrent chromatography (HPCCC) using a two-phase solvent system composed of tert-butyl methyl ether/ n-butanol/acetonitrile/water/trifluoroacetic acid (1 : 3 : 1 : 5 : 0.01, v/v) to give pure C3G (34.1 mg) and C3R (14.3 mg) from 1.5 g crude mulberry fruit extract. Using the pure C3G and C3R, a reliable high-performance liquid chromatography (HPLC) method was developed and validated to determine the C3G and C3R contents in mulberry fruit. C3G and C3R were separated simultaneously using an Eclipse XDB-C18 column (4.6 × 250 mm I.D., 5 μm) coupled with a photodiode array detector (PDA). The gradient elution of the mobile phase consisting of acetonitrile (0.5% formic acid) and water (0.5% formic acid) was applied (1.0 mL/min), and the detection wavelength was 520 nm. The calibration curves of C3G and C3R showed good linearity (both with r<sup>2</sup> = 0.9996) in the concentration range 15.625 - 500 μg/mL, and the relative standard deviations (RSD%) of intra- and interday variability were in the ranges 2.1 - 8.2% and 4.1 - 17.1%, respectively. The accuracies were ranged 96.5 - 102.6% for C3G and C3R, respectively. The developed HPLC method was used to determine the contents of C3G and C3R in newly harvested mulberry from eight different provinces of Korea.
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참고문헌 (11)
참고문헌 신청
Eo, H. J / 2014 / BMC Complement Altern. Med 14:200
Song, W / 2009 / J. Agric. Food Chem 57:9133~9140
Arfan, M / 2012 / Int. J. Mol. Sci 13:2472~2480
Kang, T. H / 2006 / Neurosci. Lett 391:122~126
Chong-De Sun / 2012 / Cyanidin-3-Glucoside-Rich Extract from Chinese Bayberry Fruit Protects Pancreatic b Cells and Ameliorates Hyperglycemia in Streptozotocin-Induced Diabetic Mice / Journal of Medicinal Food 15(3):288~298
Song, W / 2009 / J. Agric. Food Chem 57:9133~9140
Arfan, M / 2012 / Int. J. Mol. Sci 13:2472~2480
Kang, T. H / 2006 / Neurosci. Lett 391:122~126
Chong-De Sun / 2012 / Cyanidin-3-Glucoside-Rich Extract from Chinese Bayberry Fruit Protects Pancreatic b Cells and Ameliorates Hyperglycemia in Streptozotocin-Induced Diabetic Mice / Journal of Medicinal Food 15(3):288~298
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