지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 239 - 248 (10page)
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초록· 키워드
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Current approaches for the engineering of osteochondral grafts are associated with poor tissue formation and compromised integration at the interface between the cartilage and bone layers. Many researchers have attempted to provide osteochondral grafts of combined cartilage and bone for osteochondral repair to help overcome the limitations of standard procedures. Solid freeform fabrication is recognized as a promising tool for creating tissue engineering scaffolds due to advantages such as superior interconnectivity and a highly porous structure. This study aimed to develop a three-dimensional plotting system to enable the manufacturing of a biphasic graft consisting cartilage and subchondral bone for application to osteochondral defects. The material advantages of both synthetic (poly L lactide-co-polyglycolide) and natural (alginate) polymers were combined for a supporting frame and cell printing. Specifically, in order to promote the maturity of the osteochondral graft in our study, cartilage-derived ECM (cECM) or hydroxyapatate (HA) substances blended with alginate was plotted together with human fetal cartilage-derived progenitor cells in the cartilage or subchondral bone layer under a multi-nozzle deposition system. Notably, a plotted biphasic graft shows good integration between cartilage and subchondral bone layers without structural separation. Furthermore, the non-toxicity of the cECM and HA substances were proved from a live/dead assay of plotted cell-laden alginate. A fabricated osteochondral graft with cECM and HA substances showed dominant cartilage and bone tissue formation in a differentiation assay. Future studies should be done to modify the alginate physical properties for long-lasting structural stability.
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등록된 정보가 없습니다.
참고문헌 (38)
참고문헌 신청
Obradovic B / 2001 / Integration of engineered cartilage / J Orthop Res 19:1089~1097
Choi JW / 2013 / Mechanical stimulation by ultrasound enhances chondrogenic differentiation of mesenchymal stem cells in a fibrin-hyaluronic acid hydrogel / Artif Organs 37:648~655
Yang S / 2001 / The design of scaffolds for use in tissue engineering. Part I. Traditional factors / Tissue Eng 7:679~689
Holy CE / 2000 / Engineering three-dimensional bone tissue in vitro using biodegradable scaffolds : investigating initial cellseeding density and culture period / J Biomed Mater Res 51:376~382
Mano JF / 2007 / Osteochondral defects : present situation and tissue engineering approaches / J Tissue Eng Regen Med 1:261~273
Choi JW / 2013 / Mechanical stimulation by ultrasound enhances chondrogenic differentiation of mesenchymal stem cells in a fibrin-hyaluronic acid hydrogel / Artif Organs 37:648~655
Yang S / 2001 / The design of scaffolds for use in tissue engineering. Part I. Traditional factors / Tissue Eng 7:679~689
Holy CE / 2000 / Engineering three-dimensional bone tissue in vitro using biodegradable scaffolds : investigating initial cellseeding density and culture period / J Biomed Mater Res 51:376~382
Mano JF / 2007 / Osteochondral defects : present situation and tissue engineering approaches / J Tissue Eng Regen Med 1:261~273
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