지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 145 - 150 (6page)
이용수
초록· 키워드
오류제보하기
Differentiated HL-60 is an effector cell widely used for the opsonophagocytic-killing assay(OPKA) to measure efficacy of pneumococcal vaccines. We investigated the correlationbetween phenotypic expression of immunoreceptors and phagocytic ability of HL-60 cellsdifferentiated with N,N-dimethylformamide (DMF), all-trans retinoic acid (ATRA), or 1α,25-dihydroxyvitamin D3 (VitD3) for 5 days. Phenotypic change was examined by flowcytometry with specific antibodies to CD11c, CD14, CD18, CD32, and CD64. Apoptosiswas determined by flow cytometry using 7-aminoactinomycin D. Function was evaluatedby a standard OPKA against serotype 19F and chemiluminescence-based respiratory burstassay. The expression of CD11c and CD14 gradually increased upon exposure to all threeagents, while CD14 expression increased abruptly after VitD3. The expression of CD18,CD32, and CD64 increased during differentiation with all three agents. Apoptosis remainedless than 10% until day 3 but increased after differentiation by DMF or ATRA.
Differentiation with ATRA or VitD3 increased the respiratory burst after day 4. DMFdifferentiation showed a high OPKA titer at day 1 which sustained thereafter while ATRAorVitD3-differentiated cells gradually increased. Pearson analysis between the phenotypicchanges and OPKA titers suggests that CD11c might be a useful differentiation marker forHL-60 cells for use in pneumococcal OPKA.
목차
등록된 정보가 없습니다.
참고문헌 (33)
참고문헌 신청
Centers for Disease Control / 1989 / Pneumococcal polysaccharide vaccine / MMWR Morb Mortal Wkly Rep 38:64~68
Fedson DS / 2004 / Vaccines / Saunders
Whitney CG / 2000 / Active Bacterial Core Surveillance Program of the Emerging Infections Program Network. Increasing prevalence of multidrug-resistant Streptococcus pneumoniae in the United States / N Engl J Med 343:1917~1924
Tan TQ / 2003 / Antibiotic resistant infections due to Streptococcus pneumoniae : impact on therapeutic options and clinical outcome / Curr Opin Infect Dis 16:271~277
Siber GR / 1994 / Pneumococcal disease : prospects for a new generation of vaccines / Science 265:1385~1387
Fedson DS / 2004 / Vaccines / Saunders
Whitney CG / 2000 / Active Bacterial Core Surveillance Program of the Emerging Infections Program Network. Increasing prevalence of multidrug-resistant Streptococcus pneumoniae in the United States / N Engl J Med 343:1917~1924
Tan TQ / 2003 / Antibiotic resistant infections due to Streptococcus pneumoniae : impact on therapeutic options and clinical outcome / Curr Opin Infect Dis 16:271~277
Siber GR / 1994 / Pneumococcal disease : prospects for a new generation of vaccines / Science 265:1385~1387
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
CD5 Expression Dynamically Changes During the Differentiation of Human CD8+ T Cells Predicting Clinical Response to Immunotherapy
Immune Network
2023 .08
-
Extrinsic Acquisition of CD80 by Antigen-Specific CD8⁺ T Cells Regulates Their Recall Immune Responses to Acute Viral Infection
Immune Network
2019 .08
-
The Role of CD4 T Cell Help in CD8 T Cell Differentiation and Function During Chronic Infection and Cancer
Immune Network
2023 .10
-
Two Distinct Subsets Are Identified from the Peritoneal Myeloid Mononuclear Cells Expressing both CD11c and CD115
Immune Network
2019 .06
-
Leukemia propagating cells in Philadelphia chromosome-positive ALL: a resistant phenotype with an adverse prognosis
Blood Research
2018 .01
-
Prognostic Significance of CD109 Expression in Patients with Ovarian Epithelial Cancer
Journal of Pathology and Translational Medicine
2019 .01
-
CD9 Expression in Colorectal Carcinomas and Its Prognostic Significance
Journal of Pathology and Translational Medicine
2016 .01
-
Effector Memory CD8+ and CD4+ T Cell Immunity Associated with Metabolic Syndrome in Obese Children
Pediatric Gastroenterology, Hepatology & Nutrition
2021 .01
-
T-Cells Modified with CD70 as an Alternative Cellular Vaccine for Antitumor Immunity
Cancer Research and Treatment
2020 .01
-
Effect of Peripheral Blood CD4 + CD25 + Regulatory T Cell on Postoperative Immunotherapy for Patients with Renal Carcinoma
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Changes in serum levels of lipopolysaccharides and CD26 in patients with Crohn’s disease
Intestinal research
2017 .01
-
Prognostic Implication of Semi-quantitative Immunohistochemical Assessment of CD20 Expression in Diffuse Large B-Cell Lymphoma
Journal of Pathology and Translational Medicine
2016 .01
-
Stem Cell Markers Predict the Response to Sorafenib in Patients with Hepatocellular Carcinoma
Gut and Liver
2019 .01
-
Canine regulatory T cells expressing CD4+and CD25+ are proliferated by interleukin-2 cytokine
한국실험동물학회 학술발표대회 논문집
2018 .01
-
CD80CD86 deficiency disrupts regulator T cell homeostasis and induces autoimmune alopecia
한국실험동물학회 학술발표대회 논문집
2016 .08
-
Effect of IL-4 on the Development and Function of Memory-like CD8 T Cells in the Peripheral Lymphoid Tissues
Immune Network
2016 .04
-
Characterization of Human Dental Pulp Cells from Supernumerary Teeth by Using Flow Cytometry Analysis
대한소아치과학회지
2019 .01
-
Beyond the Role of CD55 as a Complement Component
Immune Network
2018 .02
-
Expression of CD73 in Breast Cancer
대한외과학회 학술대회 초록집
2017 .11
-
mTOR Signaling Combined with Cancer Stem Cell Markers as a Survival Predictor in Stage II Colorectal Cancer
Yonsei Medical Journal
2020 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0